Chee G L, Wan T S
Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon.
J Chromatogr. 1993 Jan 29;612(1):172-7. doi: 10.1016/0378-4347(93)80382-e.
The complete separation of a mixture of seventeen basic drugs of different classes was achieved with capillary zone electrophoresis in 11 min. The migration time reproducibility for individual components was between 0.5 and 1.7% relative standard deviation (R.S.D.). Peak detection was achieved by ultraviolet absorption, with peak-area reproducibility ranging from 1.5 to 6.3% R.S.D. The pH of the running buffer was critical in determining the separation of the mixture of basic drugs. The detection of most of these components in urine and plasma is also illustrated.
采用毛细管区带电泳在11分钟内实现了17种不同类别碱性药物混合物的完全分离。各组分迁移时间的重现性相对标准偏差（R.S.D.）在0.5%至1.7%之间。通过紫外吸收进行峰检测，峰面积重现性的相对标准偏差范围为1.5%至6.3%。运行缓冲液的pH值对于确定碱性药物混合物的分离至关重要。还展示了在尿液和血浆中对大多数这些组分的检测。
