Dmochowski M, Hashimoto T, Garrod D R, Nishikawa T
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
J Invest Dermatol. 1993 Apr;100(4):380-4. doi: 10.1111/1523-1747.ep12471934.
Recently, it has been shown that desmoglein, pemphigus foliaceus target antigen, and a 130-kD pemphigus vulgaris antigen belong to the cadherin family of cell adhesion molecules. We tried to determine whether desmocollins I/II, other cadherin-like transmembranous glycoproteins present in desmosomes, are also recognized by pemphigus autoantibodies of the IgG class. We examined 16 pemphigus vulgaris sera, 15 pemphigus foliaceus sera, 15 Brazilian pemphigus foliaceus sera, five bullous pemphigoid sera, and 65 normal sera. Four (25%) pemphigus vulgaris sera, one (7%) pemphigus foliaceus serum, eight (53%) Brazilian pemphigus foliaceus sera, and three (5%) normal sera reacted with desmocollins I/II on immunoblots of bovine desmosome preparation. The affinity-purified desmocollins I/II pemphigus autoantibodies were shown to bind the epidermal cell surface by indirect immunofluorescence. Immunoblot analysis revealed one pemphigus vulgaris serum, one Brazilian pemphigus foliaceus serum, and one normal serum recognizing a recombinant protein produced by a desmocollin cDNA clone. Moreover, immunoblot analysis of reactivity of a Brazilian pemphigus foliaceus serum with recombinant proteins produced by deletion mutants of the desmocollin cDNA clone showed that the extracellular portion of desmocollin is immunogenic in this pemphigus patient. We conclude that desmocollins I/II are recognized by certain sera from patients with various types of pemphigus, particularly Brazilian pemphigus foliaceus. However, the significance of this reactivity remains to be defined.
最近研究表明，落叶型天疱疮的靶抗原桥粒芯糖蛋白及寻常型天疱疮的130kD抗原属于细胞黏附分子的钙黏蛋白家族。我们试图确定桥粒芯胶蛋白I/II，即桥粒中存在的其他钙黏蛋白样跨膜糖蛋白，是否也能被IgG类天疱疮自身抗体识别。我们检测了16份寻常型天疱疮血清、15份落叶型天疱疮血清、15份巴西落叶型天疱疮血清、5份大疱性类天疱疮血清以及65份正常血清。在牛桥粒制剂的免疫印迹分析中，4份（25%）寻常型天疱疮血清、1份（7%）落叶型天疱疮血清、8份（53%）巴西落叶型天疱疮血清以及3份（5%）正常血清与桥粒芯胶蛋白I/II发生反应。亲和纯化的桥粒芯胶蛋白I/II天疱疮自身抗体通过间接免疫荧光显示能结合表皮细胞表面。免疫印迹分析显示1份寻常型天疱疮血清、1份巴西落叶型天疱疮血清以及1份正常血清能识别由桥粒芯胶蛋白cDNA克隆产生的重组蛋白。此外，对一份巴西落叶型天疱疮血清与桥粒芯胶蛋白cDNA克隆缺失突变体产生的重组蛋白的反应性进行免疫印迹分析表明，在这位天疱疮患者中，桥粒芯胶蛋白的细胞外部分具有免疫原性。我们得出结论，桥粒芯胶蛋白I/II能被各类天疱疮患者的某些血清识别，尤其是巴西落叶型天疱疮患者。然而，这种反应性的意义仍有待确定。
