Hellstrand K, Czerkinsky C, Ricksten A, Jansson B, Asea A, Kylefjord H, Hermodsson S
Department of Clinical Virology, University of Göteborg, Sweden.
J Interferon Res. 1993 Feb;13(1):33-8. doi: 10.1089/jir.1993.13.33.
Monocytes, recovered directly from peripheral blood by counter-current centrifugal elutriation (CCE), were shown to provide two regulatory signals for induction of interferon-gamma (IFN-gamma) in natural killer (NK) cells in response to interleukin-2 (IL-2): an upregulating signal and an inhibitory signal. The inhibitory signal was time-dependent, irreversible, and operating on a pretranslational level, as indicated by the inability of enriched NK cells to accumulate IFN-gamma mRNA in the presence of elutriated monocytes. Monocyte-induced inhibition of IFN-gamma production was abrogated by the biogenic amine serotonin, acting via the 5-hydroxytryptamine, or serotonin (5-HT1A), subset of serotonin receptors (5-HTR). Thereby, serotonin effectively promoted IFN-gamma production in the presence of monocytes. We conclude that serotonergic 5-HT1A receptors transduce signals that are required for NK cells to produce IFN-gamma in response to IL-2.
通过逆流离心淘析法（CCE）直接从外周血中分离得到的单核细胞，被证明能为自然杀伤细胞（NK细胞）在白细胞介素-2（IL-2）刺激下诱导产生干扰素-γ（IFN-γ）提供两种调节信号：一种上调信号和一种抑制信号。抑制信号具有时间依赖性、不可逆性，且作用于转录前水平，这表现为在存在淘析单核细胞的情况下，富集的NK细胞无法积累IFN-γ mRNA。单核细胞诱导的IFN-γ产生抑制作用可被生物胺血清素消除，血清素通过5-羟色胺或血清素受体（5-HTR）的5-羟色胺1A（5-HT1A）亚型发挥作用。因此，血清素在单核细胞存在的情况下有效地促进了IFN-γ的产生。我们得出结论，血清素能5-HT1A受体转导NK细胞在IL-2刺激下产生IFN-γ所需的信号。
