van der Vliet G M, de Wit M Y, Klatser P R
N.H. Swellengrebel Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
Mol Cell Probes. 1993 Feb;7(1):61-6. doi: 10.1006/mcpr.1993.1008.
A colorimetric assay for the detection of PCR-products is described. The assay is based on amplification of DNA in the presence of digoxigenin-dUTP. After immobilization of the PCR products to a microtitre plate, amplified DNA could be detected colorimetrically. The sensitivity of this colorimetric assay was equal to gel-analysis allowing the detection of 100 fg of template DNA. Here, we show that it can be used to detect Mycobacterium leprae DNA in biopsy specimens from leprosy patients. The simplicity and the low degree of variation make this assay an alternative to gel-analysis.
本文描述了一种用于检测PCR产物的比色测定法。该测定法基于在存在地高辛配基-dUTP的情况下对DNA进行扩增。将PCR产物固定到微量滴定板上后，可通过比色法检测扩增的DNA。这种比色测定法的灵敏度与凝胶分析相当，能够检测到100 fg的模板DNA。在此，我们表明它可用于检测麻风病患者活检标本中的麻风分枝杆菌DNA。该测定法的简单性和低变异度使其成为凝胶分析的替代方法。
