Eberspacher B E, Organisciak D T, Massaro E J
J Exp Zool. 1977 Feb;199(2):289-96. doi: 10.1002/jez.1401990213.
Liver plasma membranes of Swiss-Webster CFW mice ranging in age from 5 to 165 days were isolated employing a two-phase dextran/polyethylene glycol polymer system. Electron microscopy revealed low mitochondrial and microsomal contamination. Lipids were quantitatively extracted and subjected to two dimensional thin layer chromatography. Individual lipids were eluted and phosphorus was measured by the Malachite green method. Cholesterol was quantitated by the micro Liberman-Burchard method and sphingolipids were quantitated fluorometrically with fluorescamine. During development, phosphatidylethanolamine levels decreased from 43.2% to 19.5% whereas phosphatidylcholine levels rose from 26% to 45.2%. The ration of phospholipid/cholesterol remained essentially constant during development; but the sphingolipid/cholesterol and sphingolipid/phospholipid ratios increased from 0.75 to 1.79 and 0.11 to 0.34, respectively.
采用双相葡聚糖/聚乙二醇聚合物系统，分离了年龄在5至165天的瑞士-韦伯斯特CFW小鼠的肝细胞膜。电子显微镜检查显示线粒体和微粒体污染程度较低。对脂质进行定量提取，并进行二维薄层色谱分析。洗脱各个脂质，采用孔雀石绿法测定磷含量。采用微量利伯曼-布赫ard法对胆固醇进行定量分析，并用荧光胺对鞘脂进行荧光定量分析。在发育过程中，磷脂酰乙醇胺水平从43.2%降至19.5%，而磷脂酰胆碱水平从26%升至45.2%。在发育过程中，磷脂/胆固醇的比例基本保持恒定；但鞘脂/胆固醇和鞘脂/磷脂的比例分别从0.75增至1.79和从0.11增至0.34。
