Zoellner H, Wojta J, Gallicchio M, McGrath K, Hamilton J A
Department of Medicine, University of Melbourne, Royal Melbourne Hospital, Parkville, Vic., Australia.
Thromb Haemost. 1993 Feb 1;69(2):135-40.
Plasminogen activators are inhibited by plasminogen activator inhibitors-1 (PAI-1) and -2 (PAI-2). We describe the synthesis of PAI-2 by human vascular endothelial cells (EC) cultured from umbilical vein, saphenous vein and foreskin microvasculature in response to interleukin-1 alpha (IL-1 alpha) and tumour necrosis factor alpha (TNF alpha) and compare it with that of PAI-1. Both PAI-2 and PAI-1 were quantitated by ELISAs. PAI-2 was cell-associated while PAI-1 was secreted by EC. IL-1 alpha and TNF alpha increased the synthesis of PAI-2 and PAI-1 by EC in a dose-dependent manner. IL-1 alpha was a stronger stimulus for PAI-2 synthesis than TNF alpha, while both cytokines were equally effective for PAI-1. Northern blot analysis revealed similar changes in mRNA levels to those in antigen levels. PAI-2 synthesis by cytokine-stimulated EC may be important in thrombus formation and inflammation.
纤溶酶原激活剂受到纤溶酶原激活剂抑制剂-1（PAI-1）和-2（PAI-2）的抑制。我们描述了从人脐静脉、大隐静脉和包皮微血管培养的人血管内皮细胞（EC）在白细胞介素-1α（IL-1α）和肿瘤坏死因子α（TNFα）刺激下PAI-2的合成情况，并将其与PAI-1的合成情况进行比较。PAI-2和PAI-1均通过酶联免疫吸附测定（ELISA）进行定量。PAI-2与细胞相关，而PAI-1由内皮细胞分泌。IL-1α和TNFα以剂量依赖的方式增加内皮细胞PAI-2和PAI-1的合成。对于PAI-2的合成，IL-1α比TNFα是更强的刺激物，而对于PAI-1，这两种细胞因子的效果相同。Northern印迹分析显示，mRNA水平的变化与抗原水平的变化相似。细胞因子刺激的内皮细胞合成PAI-2可能在血栓形成和炎症中起重要作用。
