Konno S, Adachi M, Matsuura T, Sunouchi K, Hoshino H, Okazawa A, Kobayashi H, Takahashi T
First Department of Internal Medicine, Showa University School of Medicine.
Arerugi. 1993 Jan;42(1):42-7.
We examined six mouse strains, A/J, DBA/2, WBB6F1-W/Wv, WBB6F1- +/+, C3H/HeN, C57BL/6, for their bronchial reactivity to methacholine and serotonin. The mice were anaesthetized with intraperitoneal sodium pentobarbital. The trachea was intubated and one jugular vein was cannulated. Then, the mice were ventilated on air, using a small animal ventilator at a rate of 60 strokes/min with a tidal volume of 0.5 ml. Methacholine and serotonin diluted to various concentrations were administered intravenously. Bronchoconstriction was assessed by the modified Konzett-Rossler method. According to their bronchial responsiveness to methacholine and serotonin, the strains were grouped into high- (A/J, DBA/2), middle- (WBB6F1-W/Wv, -+/+) or low- (C3H/HeN, C57BL/6) responder groups.
我们检测了六种小鼠品系，即A/J、DBA/2、WBB6F1-W/Wv、WBB6F1-+/+、C3H/HeN、C57BL/6，观察它们对乙酰甲胆碱和血清素的支气管反应性。小鼠通过腹腔注射戊巴比妥钠进行麻醉。气管插管，颈静脉插管。然后，使用小动物呼吸机以60次/分钟的频率、0.5毫升的潮气量对小鼠进行通气。将稀释至不同浓度的乙酰甲胆碱和血清素静脉注射。采用改良的Konzett-Rossler方法评估支气管收缩情况。根据它们对乙酰甲胆碱和血清素的支气管反应性，这些品系被分为高反应组（A/J、DBA/2）、中反应组（WBB6F1-W/Wv、-+/+）或低反应组（C3H/HeN、C57BL/6）。
