Zhang L, Jayne D R, Oliveira D B
Department of Medicine, School of Clinical Medicine, University of Cambridge, UK.
J Autoimmun. 1993 Feb;6(1):93-105. doi: 10.1006/jaut.1993.1008.
Idiotype-anti-idiotype interactions were investigated in the sera of patients with primary biliary cirrhosis (PBC), which is characterized by the presence of circulating anti-mitochondrial antibodies (AMA). A mouse monoclonal antibody, CPZ674, has been raised to the E2 component of the pyruvate dehydrogenase complex (PDC), which is the target antigen of AMA. CPZ674 recognizes one of the epitopes recognizable by AMA, as demonstrated by competitive Western blotting. Anti-idiotypic antibodies in PBC sera were detected either by their specific binding to CPZ674 in an ELISA or by the formation of idiotype-anti-idiotype complexes with CPZ674, detected using chromatography on a Sephacryl-300 column and by a polyethylene glycol precipitation method. The specificity of the anti-idiotypic antibodies to AMA was shown by their ability to inhibit the binding of AMA to PDC, but not the binding of other autoantibodies to their relevant autoantigens. We have therefore produced evidence for the existence of idiotype-anti-idiotype interactions in PBC, but whether these anti-idiotypic antibodies are involved in the control of AMA is unknown.
在原发性胆汁性肝硬化（PBC）患者的血清中研究了独特型-抗独特型相互作用，原发性胆汁性肝硬化的特征是存在循环抗线粒体抗体（AMA）。已制备出一种针对丙酮酸脱氢酶复合体（PDC）E2成分的小鼠单克隆抗体CPZ674，而PDC是AMA的靶抗原。如竞争性蛋白质印迹法所示，CPZ674识别AMA可识别的一个表位。通过ELISA中与CPZ674的特异性结合，或通过使用Sephacryl-300柱层析和聚乙二醇沉淀法检测到的与CPZ674形成独特型-抗独特型复合物，来检测PBC血清中的抗独特型抗体。抗独特型抗体对AMA的特异性表现在其能够抑制AMA与PDC的结合，但不能抑制其他自身抗体与其相关自身抗原的结合。因此，我们已证明PBC中存在独特型-抗独特型相互作用，但这些抗独特型抗体是否参与AMA的调控尚不清楚。
