肺结核患者外周血单核细胞溶酶体酶的细胞内水平及细胞外释放

Intracellular levels and extracellular release of lysosomal enzymes from peripheral blood monocytes in pulmonary tuberculosis patients.

作者信息

Jaswal S, Dhand R, Sethi A K, Kohli K K, Ganguly N K

机构信息

Department of Internal Medicine, Experimental Medicine and Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

出版信息

APMIS. 1993 Jan;101(1):50-4. doi: 10.1111/j.1699-0463.1993.tb00080.x.

DOI:10.1111/j.1699-0463.1993.tb00080.x

PMID:8457326

Abstract

The intracellular activity and extracellular release (basal and latex-stimulated) of B-glucuronidase (BG) and N-acetylglucosaminidase (NAG), measured fluorimetrically, were observed to be significantly (P < 0.05) higher in blood monocytes (BM) of untreated pulmonary tuberculosis (TB) patients compared to those of age- and sex-matched controls and Mantoux-positive subjects without any evidence of active disease. After completion of antituberculous therapy, BG and NAG activities declined appreciably (P < 0.05) and their levels became comparable to those in control subjects. The present results suggest the potentiation of the oxygen-independent defense mechanism of BM in pulmonary TB.

摘要

通过荧光法测量发现，未经治疗的肺结核（TB）患者血液单核细胞（BM）中β-葡萄糖醛酸酶（BG）和N-乙酰氨基葡萄糖苷酶（NAG）的细胞内活性以及细胞外释放（基础和乳胶刺激），与年龄和性别匹配的对照组以及无任何活动性疾病证据的结核菌素试验阳性受试者相比，显著更高（P < 0.05）。抗结核治疗完成后，BG和NAG活性明显下降（P < 0.05），其水平与对照组相当。目前的结果表明肺结核中BM的非氧依赖性防御机制增强。