Thompson W D, Smith E B, Stirk C M, Wang J
Department of Pathology, Medical School, Aberdeen Royal Infirmary, UK.
Blood Coagul Fibrinolysis. 1993 Feb;4(1):113-5.
We have previously shown that similar patterns of fibrin degradation products (FbDP) by gel electrophoresis and immunoblotting are present in extracts of human atherosclerotic plaques, human and experimental wounds and breast cancers. Such extracts were also shown to stimulate cell proliferation including angiogenesis in the chick chorioallantoic membrane, now shown also for breast cancers. Removal of FbDP from plaque extracts by an anti-fibrinogen affinity column, or by an anti-fragment E column, reduced activity. Human FbDP prepared in vitro were active, but not FgDP. Fibrin fragment E was active, and we also showed that admixture of FbDP with a polyclonal rabbit anti-fibrin E but not anti-fibrin D neutralized activity. However attempts to raise comparable monoclonal blocking antibodies were hindered by species similarities. The response of the Balb/c mouse was predominantly directed at minor D contaminants, in contrast to the Sprague-Dawley rat which responded to fibrin fragment E in our antigen preparation.
我们之前已经表明，通过凝胶电泳和免疫印迹法检测，在人类动脉粥样硬化斑块、人类及实验性伤口和乳腺癌提取物中存在类似模式的纤维蛋白降解产物（FbDP）。这些提取物还显示出能刺激细胞增殖，包括在鸡胚绒毛尿囊膜中的血管生成，现在在乳腺癌中也得到了证实。通过抗纤维蛋白原亲和柱或抗片段E柱从斑块提取物中去除FbDP，活性会降低。体外制备的人FbDP具有活性，但FgDP没有活性。纤维蛋白片段E具有活性，并且我们还表明，将FbDP与多克隆兔抗纤维蛋白E而非抗纤维蛋白D混合可中和活性。然而，由于物种相似性，制备可比的单克隆阻断抗体的尝试受到了阻碍。与Sprague-Dawley大鼠不同，Balb/c小鼠的反应主要针对少量的D污染物，在我们的抗原制剂中，Sprague-Dawley大鼠对纤维蛋白片段E有反应。
