Janda M, Ahlquist P
Institute for Molecular Virology, University of Wisconsin, Madison 53706-1596.
Cell. 1993 Mar 26;72(6):961-70. doi: 10.1016/0092-8674(93)90584-d.
This paper shows that yeast can be a host for the replication of a higher eukaryotic viral genome. Brome mosaic virus (BMV) is a member of the alphavirus-like superfamily of animal and plant positive strand RNA viruses. Yeast expressing BMV RNA replication genes 1a and 2a support RNA-dependent replication and transcription of BMV RNA3 derivatives. RNA3 derivatives with the coat gene replaced by a reporter gene expressed that gene in a fashion dependent on 1a and 2a expression in trans and on BMV RNA replication and transcription signals in cis. RNA3 derivatives carrying the yeast URA3 gene complemented ura3- yeast to prototrophy and were maintained as persistent RNA episomes. Thus, all cellular factors essential for BMV RNA replication and transcription must be present in yeast, and yeast genetics should facilitate their identification.
本文表明酵母可以作为高等真核病毒基因组复制的宿主。雀麦花叶病毒（BMV）是动植物正链RNA病毒中类甲病毒超家族的成员。表达BMV RNA复制基因1a和2a的酵母支持BMV RNA3衍生物的RNA依赖性复制和转录。外壳基因被报告基因取代的RNA3衍生物以一种依赖于反式1a和2a表达以及顺式BMV RNA复制和转录信号的方式表达该基因。携带酵母URA3基因的RNA3衍生物使ura3 -酵母互补为原养型，并作为持久性RNA附加体得以维持。因此，BMV RNA复制和转录所必需的所有细胞因子必定存在于酵母中，并且酵母遗传学应有助于对它们的鉴定。
