Furman B L, Sneddon P
Department of Physiology and Pharmacology, University of Strathclyde, Glasgow, UK.
Eur J Pharmacol. 1993 Feb 23;232(1):29-34. doi: 10.1016/0014-2999(93)90724-v.
Endothelium-dependent and endothelium-independent responses to exogenous vasoactive substances were compared in isolated, perfused mesenteric beds from control rats and in rats subjected to prolonged (15-17 weeks) streptozotocin induced diabetes. The main aim of the study was to determine whether the prolonged period of diabetes altered vascular endothelial function, and thereby modified vascular responsiveness to vasoconstrictors or vasodilators. Noradrenaline induced vasoconstriction was not significantly altered in preparations from diabetic rats compared to control. Vasodilator responses to acetylcholine (ACh) and ATP were endothelium-dependent, since they were greatly reduced or abolished after endothelium removal by perfusion with 0.1% Triton-X 100. The vasodilator action of these agents was fully preserved in the diabetic animals. Sodium nitroprusside produced a vasodilation which was endothelium-independent, this vasodilation was also preserved in the diabetic animal. We conclude that prolonged streptozotocin-induced diabetes does not reduce the ability of the mesenteric vascular endothelium to release vasodilator substances, nor does it alter the responsiveness of the bed to exogenous endothelium-independent vasodilator sodium nitroprusside or vasoconstrictor noradrenaline.
在来自对照大鼠以及经链脲佐菌素诱导患长期（15 - 17周）糖尿病的大鼠的离体灌注肠系膜床中，比较了对外源性血管活性物质的内皮依赖性和非内皮依赖性反应。该研究的主要目的是确定长期糖尿病是否改变血管内皮功能，从而改变血管对血管收缩剂或血管扩张剂的反应性。与对照相比，去甲肾上腺素诱导的血管收缩在糖尿病大鼠的标本中没有显著改变。对乙酰胆碱（ACh）和ATP的血管舒张反应是内皮依赖性的，因为在用0.1% Triton - X 100灌注去除内皮后，它们会大大降低或消失。这些药物的血管舒张作用在糖尿病动物中完全保留。硝普钠产生的血管舒张是非内皮依赖性的，这种血管舒张在糖尿病动物中也得以保留。我们得出结论，长期链脲佐菌素诱导的糖尿病不会降低肠系膜血管内皮释放血管舒张物质的能力，也不会改变该床对外源性非内皮依赖性血管舒张剂硝普钠或血管收缩剂去甲肾上腺素的反应性。
