从牛脑细胞质中纯化巨噬细胞移动抑制因子（MIF）。
Purification of macrophage migration inhibitory factor (MIF) from bovine brain cytosol.
作者信息
Galat A, Rivière S, Bouet F
机构信息
Département d'Ingénieric et d'Etudes des Protéines (DIEP), Centre d'Etudes de Saclay (CEN-Saclay), Gif-sur-Yvette, France.
出版信息
FEBS Lett. 1993 Mar 22;319(3):233-6. doi: 10.1016/0014-5793(93)80553-7.
Two isoforms of the macrophage migration inhibitory factor (MIF) have been isolated to homogeneity from bovine brain cytosol. In agreement with the cDNA sequence of their human counterpart, they both have an apparent molecular weight of 12 kDa and are characterized by the following N-terminal amino acid sequence NH2-PMFVVNTNVPRASVPDGLLSELTQQLAQATGKPPQYIAV-. CD spectra revealed that bovine MIF contains 42% (+/- 3%) alpha-helix and 21% (+/- 3%) beta-structure. CD-constrained prediction of the secondary structure assigned MIF to the alpha/beta-class of proteins.
已从牛脑细胞质中分离出两种同质性的巨噬细胞迁移抑制因子（MIF）同工型。与它们人类对应物的cDNA序列一致，它们的表观分子量均为12 kDa，其特征在于以下N端氨基酸序列：NH2-PMFVVNTNVPRASVPDGLLSELTQQLAQATGKPPQYIAV-。圆二色光谱显示，牛MIF含有42%（±3%）的α-螺旋和21%（±3%）的β-结构。基于圆二色光谱的二级结构预测将MIF归类为α/β类蛋白质。
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