Koslovsky J S, Qian C, Jiang X, Mercer J A
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235-9040.
FEBS Lett. 1993 Apr 5;320(2):121-4. doi: 10.1016/0014-5793(93)80075-6.
We have isolated two cDNAs that encode putative myosin I heavy chains by polymerase chain reaction amplification of brain cDNA with degenerate oligodeoxynucleotide primers representing myosin I-specific conserved amino acid sequences. We report the complete deduced amino acid sequence of one of these cDNAs. The sequences is most similar to those of the avian and bovine brush border myosin Is, with five putative calmodulin-binding repeats at the head-tail junction. Northern analysis demonstrates that this myosin heavy chain, unlike the brush border myosins, is expressed in many tissues.
我们通过聚合酶链反应扩增脑cDNA，使用代表肌球蛋白I特异性保守氨基酸序列的简并寡脱氧核苷酸引物，分离出了两个编码假定肌球蛋白I重链的cDNA。我们报告了其中一个cDNA完整推导的氨基酸序列。该序列与禽和牛的刷状缘肌球蛋白I的序列最为相似，在头尾交界处有五个假定的钙调蛋白结合重复序列。Northern分析表明，这种肌球蛋白重链与刷状缘肌球蛋白不同，在许多组织中都有表达。
