Abdel Malak C A, Lavrenova G I, Lysogorskaya E N, Stepanov V M
Chemistry Department, M.V. Lomonosov Moscow State University, Russia.
Int J Pept Protein Res. 1993 Feb;41(2):97-101. doi: 10.1111/j.1399-3011.1993.tb00119.x.
Swine pepsin at pH 5 efficiently catalyzes a condensation between Z-Ala-Ala-Phe-OH and p-nitroanilides of Leu, Phe, Val, Ala and Arg that leads to formation of corresponding benzyloxycarbonyl-tetrapeptide p-nitroanilides with yields of 70-90%. These reactions are complicated by co-precipitation of pepsin and the reaction products that necessitates the use of a relatively high concentration of pepsin.
在pH 5的条件下，猪胃蛋白酶能有效地催化Z - 丙氨酰 - 丙氨酰 - 苯丙氨酸 - 羟基（Z - Ala - Ala - Phe - OH）与亮氨酸、苯丙氨酸、缬氨酸、丙氨酸和精氨酸的对硝基苯胺形成缩合反应，生成相应的苄氧羰基 - 四肽对硝基苯胺，产率为70 - 90%。这些反应因胃蛋白酶与反应产物的共沉淀而变得复杂，这就需要使用相对较高浓度的胃蛋白酶。
