Turman M A, Yabe T, McSherry C, Bach F H, Houchins J P
Department of Pediatrics, University of Minnesota, Minneapolis 55455.
Hum Immunol. 1993 Jan;36(1):34-40. doi: 10.1016/0198-8859(93)90006-m.
NKG7 is a cDNA clone generated from a human NK-cell clone. The DNA and predicted aa sequence of NKG7 is not homologous with any previously reported genes or peptides. NKG7 mRNA is expressed in activated T cells and in A-LAK cells isolated from the peripheral blood of normal individuals, and in normal human kidney, liver, lung and pancreas. Furthermore, NKG7 mRNA is expressed at high levels in TCR gamma delta-expressing CTL clones, and in some TCR alpha beta-expressing CTL clones (both CD4+ and CD8+), but is not expressed in other TCR alpha beta-expressing CTL clones and in cell lines representing B cells, monocytes, and myeloid cells. NKG7 mRNA is not expressed in normal human brain, heart, or skeletal muscle. Southern hybridization of NKG7 suggests that NKG7 is a single-copy gene localized to chromosome 19. A hydropathicity profile of the predicted 148 aa polypeptide indicates that NKG7 is a type-I integral membrane protein with a 38-aa extracellular domain and a 61-aa cytoplasmic domain. These results indicate that the NKG7 gene encodes a novel cell surface protein expressed in several cell types, including NK cells and T cells.
NKG7是从一个人自然杀伤细胞克隆体中产生的cDNA克隆。NKG7的DNA和预测的氨基酸序列与任何先前报道的基因或肽均无同源性。NKG7信使核糖核酸在活化的T细胞、从正常个体外周血中分离出的A-LAK细胞以及正常人的肾脏、肝脏、肺和胰腺中表达。此外，NKG7信使核糖核酸在表达TCRγδ的细胞毒性T淋巴细胞克隆以及一些表达TCRαβ的细胞毒性T淋巴细胞克隆（CD4+和CD8+）中高水平表达，但在其他表达TCRαβ的细胞毒性T淋巴细胞克隆以及代表B细胞、单核细胞和髓样细胞的细胞系中不表达。NKG7信使核糖核酸在正常人的脑、心脏或骨骼肌中不表达。NKG7的Southern杂交表明NKG7是一个定位于19号染色体的单拷贝基因。预测的148个氨基酸多肽的亲水性图谱表明NKG7是一种I型整合膜蛋白，具有一个38个氨基酸的细胞外结构域和一个61个氨基酸的细胞质结构域。这些结果表明NKG7基因编码一种在包括自然杀伤细胞和T细胞在内的几种细胞类型中表达的新型细胞表面蛋白。
