光动力对人皮肤成纤维细胞核膜的影响。

Photodynamic effects on the nuclear envelope of human skin fibroblasts.

作者信息

Krammer B, Hubmer A, Hermann A

机构信息

Department of Biophysics, University of Salzburg, Austria.

出版信息

J Photochem Photobiol B. 1993 Feb;17(2):109-14. doi: 10.1016/1011-1344(93)80003-r.

DOI:10.1016/1011-1344(93)80003-r

PMID:8459315

Abstract

Photodynamic effects on the nuclear envelope of human skin fibroblasts were investigated by confocal laser fluorescence microscopy and transmembrane resting potential measurements. The results show staining of the nuclear envelope after short incubation times with Photosan III, Photofrin II and haematoporphyrin derivative (HPD) enriched with monomers. Maximum staining was found at the centre of the nuclear envelope. The sequence of fluorescence intensity was HPD enriched with monomers > Photofrin II > Photosan III. After lethal treatment with Photosan III and tetrasulphonated aluminium chloride phthalocyanine, the nuclear transmembrane potential of the nuclear membrane decreased from -20 mV to about -10 mV with reference to the plasma membrane potential.

摘要

通过共聚焦激光荧光显微镜和跨膜静息电位测量，研究了光动力对人皮肤成纤维细胞核膜的影响。结果显示，在与富含单体的光森三号、卟吩姆钠和血卟啉衍生物（HPD）短时间孵育后，核膜出现染色。在核膜中心发现最大染色。荧光强度顺序为富含单体的HPD＞卟吩姆钠＞光森三号。在用光森三号和四磺化铝酞菁进行致死处理后，相对于质膜电位，核膜的核跨膜电位从-20mV降至约-10mV。