斯氏舌蝇坦桑尼亚种群的遗传变异（双翅目：舌蝇科）

Genetic variation in a Tanzanian population of Glossina swynnertoni (Diptera: Glossinidae).

作者信息

Gooding R H, Mbise S, Macha P, Rolseth B M

机构信息

Department of Entomology, University of Alberta, Edmonton, Canada.

出版信息

J Med Entomol. 1993 Mar;30(2):489-92. doi: 10.1093/jmedent/30.2.489.

DOI:10.1093/jmedent/30.2.489

PMID:8459430

Abstract

Adult Glossina swynnertoni Austen that emerged from puparia collected during 1989 and 1991 near Makuyuni, Tanzania, were examined by polyacrylamide gel electrophoresis. Fourteen of 17 enzymes were monomorphic. Midgut alkaline phosphatase (ALKPH), phosphoglucomutase (PGM), and glucose-6-phosphate isomerase (PGI) from the head and thorax were polymorphic. Banding patterns indicated that the locus for PGM was on the X chromosome and loci for ALKPH and PGI were autosomal. For the 17 loci studied, the mean heterozygosity per locus was 6.1 +/- 3.7% in the 1989 sample and 5.7 +/- 3.7% in the 1991 sample. The effective number of alleles per locus was 1.11 and 1.10 in these samples. This level of genetic variation was low compared with other populations of tsetse flies and indicated that the sample may have been drawn from a small inbred population or one that recently had gone through a genetic bottleneck.

摘要

对1989年和1991年在坦桑尼亚马库尤尼附近采集的蛹羽化出的成年斯温氏舌蝇（Glossina swynnertoni Austen）进行了聚丙烯酰胺凝胶电泳分析。17种酶中有14种是单态的。头部和胸部的中肠碱性磷酸酶（ALKPH）、磷酸葡萄糖变位酶（PGM）和葡萄糖-6-磷酸异构酶（PGI）是多态的。条带模式表明，PGM基因座位于X染色体上，ALKPH和PGI基因座位于常染色体上。对于所研究的17个基因座，1989年样本中每个基因座的平均杂合度为6.1±3.7%，1991年样本中为5.7±3.7%。这些样本中每个基因座的有效等位基因数分别为1.11和1.10。与其他采采蝇种群相比，这种遗传变异水平较低，表明该样本可能取自一个小型近交种群或最近经历了遗传瓶颈的种群。