Kleene S J
Department of Anatomy and Cell Biology, University of Cincinnati, OH 45267-0521.
J Neurosci Methods. 1993 Jan;46(1):11-6. doi: 10.1016/0165-0270(93)90136-f.
A simple method is described for achieving a salt bridge within a patch pipette. The tip of the pipette is filled with a Cl(-)-free solution that bathes the membrane patch. Above this is a Cl(-)-containing solution that surrounds the recording Ag/AgCl wire electrode. Addition of 0.07% (w/v) agarose to the solution in the tip prevents bulk mixing of the 2 phases within the pipette. This configuration makes it possible to remove Cl- from the membrane surface but maintain it at the electrode surface. The presence of the Cl(-)-free phase in the bottom of the patch pipette does not reduce the electrical stability of the recording electrode.
本文描述了一种在膜片吸管内实现盐桥的简单方法。吸管尖端填充有无Cl⁻溶液，该溶液浸没膜片。在此之上是含Cl⁻溶液，其包围记录用的Ag/AgCl线电极。向尖端溶液中添加0.07%（w/v）琼脂糖可防止吸管内两相的大量混合。这种配置使得能够从膜表面去除Cl⁻，但在电极表面维持Cl⁻。膜片吸管底部无Cl⁻相的存在不会降低记录电极的电稳定性。
