Ota A, Morishita H
School of Medicine, University of Osaka, Japan.
Microbios. 1993;73(295):149-55.
The effect of NaCl on mycelial growth of Saccharomycopsis fibuligera was examined. Cells were able to grow in medium containing 1 M or 2 M NaCl after adaptation to the salt under standing culture conditions. It took more time to adapt to 2 M NaCl than to 1 M NaCl. Hollow-looking regions in the cells increased during incubation for 20 days in medium without salt, but diminished in cells incubated in medium containing either of the two concentrations of NaCl. The mycelial cells incubated in the medium containing salt were slimmer, and bubble shaped, and malformed cells were observed. The extent of the change was greater in 2 M NaCl than that in 1 M NaCl. NaCl protected cells from leaking, and produced malformed cells.
研究了氯化钠对扣囊复膜孢酵母菌丝体生长的影响。在静置培养条件下适应盐分后,细胞能够在含有1 M或2 M氯化钠的培养基中生长。适应2 M氯化钠比适应1 M氯化钠需要更多时间。在无盐培养基中培养20天期间,细胞中出现的中空区域增加,但在含有两种浓度氯化钠之一的培养基中培养的细胞中,中空区域减少。在含盐培养基中培养的菌丝体细胞更细长,呈泡状,并且观察到细胞畸形。2 M氯化钠中的变化程度大于1 M氯化钠中的变化程度。氯化钠可保护细胞不发生渗漏,并产生畸形细胞。