对体细胞杂种中失活小鼠X染色体的评估。

An evaluation of the inactive mouse X chromosome in somatic cell hybrids.

作者信息

Salido E C, Passage M B, Yen P H, Shapiro L J, Mohandas T K

机构信息

Department of Pediatrics, University of California, San Francisco 94143.

出版信息

Somat Cell Mol Genet. 1993 Jan;19(1):65-71. doi: 10.1007/BF01233955.

DOI:10.1007/BF01233955

PMID:8460399

Abstract

The expression of mouse Zfx, Rps4, Ube1x, and Xist was evaluated in hamster-mouse somatic cell hybrids containing either an active or an inactive mouse X chromosome using polymerase chain reaction of reverse transcribed RNA (RT-PCR). The results showed that Zfx, Rps4, and Ube1x are expressed exclusively from the active mouse X, while Xist is expressed exclusively from the inactive X. These findings confirm the pattern of X inactivation for these mouse genes reported previously based on expression in somatic tissues of F1 females from interspecific crosses. These results demonstrate the existence of differences between human and mouse X inactivation, as the corresponding human genes, ZFX, RPS4X, and UBE1 escape X inactivation.

摘要

利用逆转录RNA的聚合酶链反应（RT-PCR），在含有活跃或不活跃小鼠X染色体的仓鼠-小鼠体细胞杂种中评估了小鼠Zfx、Rps4、Ube1x和Xist的表达。结果显示，Zfx、Rps4和Ube1x仅从活跃的小鼠X染色体表达，而Xist仅从不活跃的X染色体表达。这些发现证实了先前基于种间杂交F1雌性体细胞组织中的表达所报道的这些小鼠基因的X染色体失活模式。这些结果表明人类和小鼠的X染色体失活存在差异，因为相应的人类基因ZFX、RPS4X和UBE1逃避X染色体失活。