核糖体蛋白S4基因定位于小鼠X染色体失活中心附近。

Rps4 maps near the inactivation center on the mouse X chromosome.

作者信息

Hamvas R M, Zinn A, Keer J T, Fisher E M, Beer-Romero P, Brown S D, Page D C

机构信息

Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, London, United Kingdom.

出版信息

Genomics. 1992 Feb;12(2):363-7. doi: 10.1016/0888-7543(92)90386-7.

DOI:10.1016/0888-7543(92)90386-7

PMID:1740345

Abstract

RPS4Y, a Y-linked gene in humans, appears to encode an isoform of ribosomal protein S4. A homologous locus on the human X chromosome, RPS4X, lies close to the X-inactivation center but fails to undergo X-inactivation. We have isolated a genomic clone from the mouse Rps4 locus, the homolog of human RPS4X. We derived an intron probe that hybridizes to the functional Rps4 locus but does not cross-hybridize to related sequences elsewhere in the mouse genome. Genetic mapping utilizing interspecific mouse backcrosses and the intron-specific probe demonstrates that Rps4 maps close to the Phka locus on the mouse X chromosome and in the vicinity of the X-inactivation center. The gene order Ccg-1-Rps4/Phka-Xist-Pgk-1 is conserved between mouse and human.

摘要

RPS4Y是人类的一个Y连锁基因，似乎编码核糖体蛋白S4的一种异构体。人类X染色体上的一个同源位点RPS4X，靠近X染色体失活中心，但不会发生X染色体失活。我们从小鼠Rps4位点（人类RPS4X的同源物）分离出了一个基因组克隆。我们获得了一个内含子探针，它与功能性Rps4位点杂交，但不会与小鼠基因组其他地方的相关序列交叉杂交。利用种间小鼠回交和内含子特异性探针进行的基因定位表明，Rps4定位于小鼠X染色体上靠近Phka位点且在X染色体失活中心附近。小鼠和人类之间的基因顺序Ccg-1-Rps4/Phka-Xist-Pgk-1是保守的。