Robinson J W, Mirkovitch V, Gomba S
Kidney Int. 1977 Feb;11(2):86-92. doi: 10.1038/ki.1977.13.
Dog kidneys were subjected to one, two, or three hours' normothermic ischemia in situ and were then excised for biochemical and histological evaluation. The uptake of para-aminohippurate (PAH) by cortical slices progressively decreased with prolongation of the ischemia, but active transport was never abolished. Glycine uptake and oxygen consumption were only reduced to a modest extent by the ischemia. The intracellular ion levels were drastically altered, with loss of potassium and gain of sodium and chloride, and considerable increases in tissue water were observed. Acid phosphatase was liberated by the whole organ into the venous blood and by the incubated slices into the incubation medium, but both biochemical and histochemical techniques showed that the total quantity of the enzyme in the cells was hardly changed. The histochemical reaction product was localized exclusively in the lysosomes. Morphological damage was slight after one or two hours' ischemia, but more pronounced after three hours, when some cells were seen to be detached from the basement membrane. These relatively minor changes seem insufficient to predict the ultimate fate of the organ after ischemia.
对狗的肾脏进行1小时、2小时或3小时的原位常温缺血处理，然后切除进行生化和组织学评估。随着缺血时间延长，皮质切片对对氨基马尿酸（PAH）的摄取逐渐减少，但主动转运从未被消除。缺血仅使甘氨酸摄取和氧消耗适度降低。细胞内离子水平发生剧烈变化，钾丢失，钠和氯增加，且观察到组织水分显著增加。酸性磷酸酶由整个器官释放入静脉血中，由孵育切片释放入孵育培养基中，但生化和组织化学技术均表明细胞内该酶的总量几乎没有变化。组织化学反应产物仅定位于溶酶体中。缺血1或2小时后形态学损伤轻微，但3小时后更明显，此时可见一些细胞从基底膜脱落。这些相对较小的变化似乎不足以预测器官缺血后的最终命运。
