Dettin M, De Rossi A, Autiero M, Guardiola J, Chieco-Bianchi L, Di Bello C
Institute of Industrial Chemistry, University of Padua, Italy.
Biochem Biophys Res Commun. 1993 Mar 15;191(2):364-70. doi: 10.1006/bbrc.1993.1226.
In previous studies we have demonstrated that synthetic peptides, corresponding to sequences in the (307-330) region of the gp120 principal neutralizing domain of different HIV-1 isolates are specifically recognized by a site distinct from the high affinity gp120-binding site of CD4. Interestingly, a peptide designed from the HIV-1 MN strain is able to enhance viral infection, while a HTLV-IIIB derived analogue is at least ten-fold less efficient and no effect is shown by other tested peptides. This enhancing effect occurs in the early step of infection and it is not strain restricted. A correlation between structure and biological functions evidenced by CD, FT-IR, and preliminary mono and bidimensional NMR is presented in this paper. The experimental data are compared to the predictions obtained by theoretical calculations.
在先前的研究中，我们已经证明，与不同HIV-1分离株的gp120主要中和结构域（307-330）区域中的序列相对应的合成肽，能被一个不同于CD4高亲和力gp120结合位点的位点特异性识别。有趣的是，一种从HIV-1 MN株设计的肽能够增强病毒感染，而一种源自HTLV-IIIB的类似物的效率至少低十倍，其他测试肽则没有显示出效果。这种增强作用发生在感染的早期阶段，且不受毒株限制。本文介绍了通过圆二色光谱（CD）、傅里叶变换红外光谱（FT-IR）以及初步的一维和二维核磁共振（NMR）所证明的结构与生物学功能之间的相关性。将实验数据与理论计算得到的预测结果进行了比较。
