Itoh K, Nishimoto I
Institute of Environmental Science and Technology, Yokohama National University, Japan.
Biochem Biophys Res Commun. 1993 Mar 15;191(2):447-52. doi: 10.1006/bbrc.1993.1238.
We have applied discharge plasma to the polishing of micropipettes for patch clamp experiments instead of the conventional heat-polishing method. Using the micropipettes given the treatment with O2 plasma, we succeeded in the formation of giga-seals on human Jurkat cells with 70% of trials. Ellipsometrical measurement of the effect of O2 plasma to etch thin films of SiO2 suggested that O2 plasma has virtually no effect on the etching of glass surface. Multiple advantages of this novel method for polishing are noted relative to the conventional method. In particular, this method makes the micropipette polishing remarkably convenient. The polishing effect of O2 plasma on micropipettes without effect on the physical etching provides a novel insight into the mechanism of polishing.
我们已将放电等离子体应用于膜片钳实验中微量移液器的抛光，而非传统的热抛光方法。使用经过氧气等离子体处理的微量移液器，我们在70%的实验中成功地在人Jurkat细胞上形成了千兆欧封接。椭圆偏振测量氧气等离子体对二氧化硅薄膜的蚀刻效果表明，氧气等离子体实际上对玻璃表面的蚀刻没有影响。相对于传统方法，这种新型抛光方法具有多个优点。特别是，这种方法使微量移液器的抛光非常方便。氧气等离子体对微量移液器的抛光效果且对物理蚀刻无影响，为抛光机制提供了新的见解。
