Chrambach A, Aldroubi A
Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Electrophoresis. 1993 Jan-Feb;14(1-2):18-22. doi: 10.1002/elps.1150140104.
The efficiency of size separations of polystyrene sulfate, 120-1085 nm radius, by molecular sieving in polymer solutions, expressed by the separation efficiency function S = M0 magnitude of dKR(R)/dR Te-KR(R)T [where M0 is the mobility in free solution, KR the retardation coefficient, R the geometric mean radius of the particle, KR(R) the retardation coefficient as a function of R, and the polymer concentration is T] increases from methylhydroxypropyl cellulose to polyvinyl alcohol to uncrosslinked polyacrylamide to agarose above its gelling temperature. Separations of DNA, in the size range of 3-21 nm radius, are by at least one order of magnitude more efficient than those of polystyrene sulfate in the size range of 120-1085 nm radius. A plot of S vs. R in the experimental range of T is constructed for the four polymer solutions; this allows one to select optimal media and concentrations for the sieving of particles in the desired range of molecular sizes.
通过在聚合物溶液中进行分子筛分离来分离半径为120 - 1085纳米的聚苯乙烯磺酸盐，其分离效率由分离效率函数S = M0 |dKR(R)/dR| Te-KR(R)T表示[其中M0是在自由溶液中的迁移率，KR是阻滞系数，R是颗粒的几何平均半径，KR(R)是作为R的函数的阻滞系数，聚合物浓度为T]，从甲基羟丙基纤维素到聚乙烯醇，再到未交联的聚丙烯酰胺，直至高于其凝胶化温度的琼脂糖，该效率逐渐增加。半径在3 - 21纳米范围内的DNA分离效率比半径在120 - 1085纳米范围内的聚苯乙烯磺酸盐至少高一个数量级。针对这四种聚合物溶液，在T的实验范围内绘制了S与R的关系图；这使得人们能够为在所需分子尺寸范围内筛分颗粒选择最佳的介质和浓度。
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