Scanlon A R, Sunderland S J, Martin T L, Goulding D, O'Callaghan D, Williams D H, Headon D R, Boland M P, Ireland J J, Roche J F
Agricultural and Veterinary Biotechnology Programme, University College, Dublin, Ireland.
J Reprod Fertil. 1993 Jan;97(1):213-22. doi: 10.1530/jrf.0.0970213.
Two experiments were conducted in cyclic beef heifers to determine whether active immunization against bovine inhibin alpha 1-26 Gly-Tyr (bINH) affected follicular dynamics, hormone concentration or ovulation rate. In Expt 1, heifers (n = 9) were actively immunized against bINH conjugated to human alpha globulins (HAG) using bis-diazotized benzidine in non-ulcerative Freund's adjuvant (NUFA; primary on day 0; booster injections on days 53, 84 and 116 using conjugated bINH and on days 176 and 366 using unconjugated bINH; ten heifers were used as controls). Ovaries were examined daily using ultrasound scanning (days 70-155 and 384-391) and corresponding blood samples were collected for bINH antibody titre, luteinizing hormone (LH), follicle-stimulating hormone (FSH) and oestradiol determinations. Four treated and four control heifers were injected with 10 micrograms gonadotrophin-releasing hormone (GnRH) on day 386 (day 2 of the oestrous cycle). Although bINH-immunized heifers had variable antibody titres ranging from 4 to 50% I125-labelled bINH bound to serum diluted 1:2000, ovulation rate was unaffected. In oestrous cycles with three dominant follicles, the ovulatory follicles grew faster (2.5 +/- 0.2 versus 1.6 +/- 0.3 mm day-1; mean +/- SEM), had shorter durations of growth (5.7 +/- 0.8 versus 9.6 +/- 1.6 days) and duration of detection (7.5 +/- 0.8 versus 12.0 +/- 2.4 days) in immunized heifers. Mean concentrations of FSH, LH and oestradiol were unaltered in most cases during oestrous cycles in bINH-immunized compared with control heifers. There was no significant difference in the percentage increase in FSH or LH, after GnRH injection, between control and immunized heifers. As ovulation rate was unaltered in the first experiment, a second similar study was designed using a different immunization protocol. In Expt 2, heifers were immunized with bINH conjugated to human serum albumin using glutaraldehyde with the following doses: 0.0 (control; n = 7), 0.33 (n = 7), 1.0 (n = 8) and 3.0 (n = 7) mg. Three booster immunizations were given 33, 66 and 209 days after primary immunization. Immunization increased the number of oestrous cycles with multiple ovulations (42 of 132 (32%) oestrous cycles examined) compared with controls (1 of 30 (3.3%) oestrous cycles examined). Neither titre nor ovulation rate was affected by dose of bINH used. In summary, following bINH immunization, ovulation rate was not increased despite changes in follicular dynamics in Expt 1, but was increased in 32% of oestrous cycles in Expt 2.(ABSTRACT TRUNCATED AT 400 WORDS)
进行了两项试验，以确定对牛抑制素α1 - 26甘氨酸 - 酪氨酸（bINH）进行主动免疫是否会影响卵泡动态、激素浓度或排卵率。在试验1中，使用双偶氮联苯胺将bINH与人α球蛋白（HAG）偶联，在非溃疡性弗氏佐剂（NUFA）中对9头小母牛进行主动免疫（第0天初次免疫；第53、84和116天使用偶联的bINH进行加强注射，第176和366天使用未偶联的bINH进行加强注射；10头小母牛作为对照）。在第70 - 155天和第384 - 391天每天使用超声扫描检查卵巢，并采集相应的血样以测定bINH抗体滴度、促黄体生成素（LH）、促卵泡生成素（FSH）和雌二醇。在第386天（发情周期第2天），对4头经处理的小母牛和4头对照小母牛注射10微克促性腺激素释放激素（GnRH）。尽管bINH免疫的小母牛抗体滴度变化范围为与稀释1:2000的血清结合的125I标记bINH的4%至50%，但排卵率未受影响。在有3个优势卵泡的发情周期中，免疫的小母牛排卵卵泡生长更快（2.5±0.2对1.6±0.3毫米/天；平均值±标准误），生长持续时间更短（5.7±0.8对9.6±1.6天），检测持续时间更短（7.5±0.8对12.0±2.4天）。与对照小母牛相比，在bINH免疫的小母牛发情周期的大多数情况下，FSH、LH和雌二醇的平均浓度未改变。GnRH注射后，对照小母牛和免疫小母牛之间FSH或LH的百分比增加没有显著差异。由于在第一个试验中排卵率未改变，因此设计了第二个类似的研究，采用不同的免疫方案。在试验2中，使用戊二醛将bINH与人血清白蛋白偶联对小母牛进行免疫，剂量如下：0.0（对照；n = 7）、0.33（n = 7）、1.0（n = ）和3.0（n = 7）毫克。在初次免疫后33、66和209天进行三次加强免疫。与对照（检查的30个发情周期中的1个（3.3%））相比，免疫增加了多排卵的发情周期数量（检查的132个发情周期中的42个（32%））。使用的bINH剂量既不影响滴度也不影响排卵率。总之，在bINH免疫后，尽管试验1中卵泡动态发生了变化，但排卵率并未增加，但在试验2的32%的发情周期中排卵率增加了。（摘要截断于400字）
