Jansen J H, Fibbe W E, Wientjens G J, Willemze R, Kluin-Nelemans J C
Department of Hematology, University Medical Center, Leiden, The Netherlands.
Leukemia. 1993 Apr;7(4):643-5.
Since interleukin-4 (IL-4) specifically inhibits monocytic colony formation in human bone marrow cultures, we investigated whether a similar inhibition could be observed in cultures of optimally stimulated acute myeloid leukemia cells with myelomonocytic differentiation (AML-M4/M5). Sensitivity to IL-4 was tested in 19 cases of AML-M4/M5, using both a 3H-thymidine incorporation assay and a clonogenic assay. Proliferation was stimulated with a combination of IL-3, granulocyte-monocyte colony-stimulating factor (GM-CSF), and conditioned medium from phytohemagglutinin-stimulated leukocytes (PHA-CM). In 13 out of 14 evaluable cases, IL-4 inhibited 3H-thymidine incorporation; stimulation was seen in one case. Using a clonogenic assay, IL-4 inhibited colony formation in all evaluable cases (n = 7). Addition of IL-6 did not alter the observed inhibition by IL-4 in 9 out of 10 cases tested. We conclude that IL-4 inhibits the proliferation of optimally stimulated AML-M4/M5 cells in most cases tested, and that this effect is not generally caused by inhibition of autocrine IL-6 production.
由于白细胞介素-4（IL-4）能特异性抑制人骨髓培养中的单核细胞集落形成，我们研究了在具有髓单核细胞分化能力的最佳刺激急性髓系白血病细胞（AML-M4/M5）培养物中是否能观察到类似的抑制作用。使用3H-胸腺嘧啶核苷掺入试验和克隆形成试验，对19例AML-M4/M5患者进行了IL-4敏感性测试。用IL-3、粒细胞-单核细胞集落刺激因子（GM-CSF）和植物血凝素刺激的白细胞条件培养基（PHA-CM）联合刺激细胞增殖。在14例可评估病例中的13例中，IL-4抑制了3H-胸腺嘧啶核苷掺入；仅1例观察到刺激作用。使用克隆形成试验，在所有可评估病例（n = 7）中，IL-4均抑制了集落形成。在10例测试病例中的9例中，添加IL-6并未改变IL-4所观察到的抑制作用。我们得出结论，在大多数测试病例中，IL-4抑制最佳刺激的AML-M4/M5细胞的增殖，并且这种作用通常不是由自分泌IL-6产生的抑制所引起的。
