Siitonen T, Zheng A, Savolainen E R, Koistinen P
Department of Internal Medicine, University of Oulu, Finland.
Ann Hematol. 1995 Apr;70(4):203-7. doi: 10.1007/BF01700376.
The effect of interleukin-4 (IL-4) on peripheral blood (PB) granulocyte-macrophage (GM) progenitors was investigated in the presence and absence of other hematopoietic growth factors, especially the mast cell growth factor (MGF), in eight healthy controls and in 26 patients with myeloproliferative disorders (MPDs) using a clonogenic cell culture assay. In the controls IL-4 was effective alone, stimulating myeloid colony growth in 50%, while MGF had no effect as a single factor. When either IL-4 or MGF was added to the combination of IL-3, GM-CSF, G-CSF, and IL-6, a statistically significant increase in the colony number was observed. The most potent colony formation took place when all these GFs were combined. In the combinations, the effect of IL-4 was additive, while MGF worked synergistically. In the MPDs, IL-4 had no effect at all on the GM progenitors in the whole group of MPDs or on the different subgroups.
采用克隆形成细胞培养试验，在8名健康对照者和26名骨髓增殖性疾病（MPD）患者中，研究了白细胞介素-4（IL-4）在存在和不存在其他造血生长因子（尤其是肥大细胞生长因子（MGF））的情况下对外周血（PB）粒细胞-巨噬细胞（GM）祖细胞的影响。在对照中，IL-4单独有效，刺激50%的髓系集落生长，而MGF作为单一因子无作用。当将IL-4或MGF添加到IL-3、GM-CSF、G-CSF和IL-6的组合中时，观察到集落数有统计学意义的增加。当所有这些生长因子联合使用时，集落形成最有效。在这些组合中，IL-4的作用是相加的，而MGF起协同作用。在MPD中，IL-4对整个MPD组或不同亚组的GM祖细胞完全没有作用。
