Yoshida A, Asaga T, Masuzawa C, Kawahara S, Yanoma S, Shimizu A, Harada M
Department of Surgery, Kanagawa Cancer Center, Yokohama, Japan.
Nihon Geka Gakkai Zasshi. 1993 Feb;94(2):177-81.
Recently, nuclear DNA contents of various human tumors were studied, and DNA aneuploidy was thought to have prognostic significance in many kinds of malignant tumors. Although the same significance was reported in thyroid carcinoma, the anaplastic thyroid carcinoma which is one of the most aggressive tumor in human malignant neoplasms, not always shows DNA aneuploidy. Therefore, using 3 xenografts established from 3 patients with anaplastic thyroid carcinomas, we investigated their growth activity, DNA ploidy and chromosome abnormalities. Two of these xenografts grew relatively fast in nude mice showing diploid or near diploid state in a flow cytometric study, and also, showed many structural abnormalities in chromosome analysis by the G-banding technique. The remaining one xenograft showed slower growth and aneuploidy, and had extensive numerical but less structural variability in its chromosomal constitution. These results indicate that some tumors showed DNA diploidy and have structural chromosome abnormalities and also suggest that the prognostic value of quantitative DNA measurement is limited in such tumors.
最近，对各种人类肿瘤的核DNA含量进行了研究，并且DNA非整倍体被认为在多种恶性肿瘤中具有预后意义。尽管甲状腺癌也有同样的意义报道，但间变性甲状腺癌是人类恶性肿瘤中最具侵袭性的肿瘤之一，并非总是表现出DNA非整倍体。因此，我们利用从3例间变性甲状腺癌患者身上建立的3个异种移植瘤，研究了它们的生长活性、DNA倍性和染色体异常情况。其中两个异种移植瘤在裸鼠中生长相对较快，流式细胞术研究显示为二倍体或近二倍体状态，并且在通过G带技术进行的染色体分析中也显示出许多结构异常。其余一个异种移植瘤生长较慢且为非整倍体，其染色体组成具有广泛的数量变异但结构变异较少。这些结果表明，一些肿瘤表现出DNA二倍体并具有染色体结构异常，并且还提示在这类肿瘤中定量DNA测量的预后价值有限。
