来自十个可变外显子的剪接选择决定了CD44的变异性。
Splicing choice from ten variant exons establishes CD44 variability.
作者信息
Tölg C, Hofmann M, Herrlich P, Ponta H
机构信息
Kernforschungszentrum Karlsruhe, Institut für Genetik, Germany.
出版信息
Nucleic Acids Res. 1993 Mar 11;21(5):1225-9. doi: 10.1093/nar/21.5.1225.
Abstract
The enormous heterogeneity of the surface protein designated CD44 is in part due to posttranslational modification, and in part due to differential splicing. Alternative splicing occurs within one particular region encoding the extracellular portion of the protein. Comparison of various cDNA clones with different 'inserts' in this variable region with sequences of genomic clones from the mouse has revealed the existence of at least ten exons from which sequences are chosen by alternative splicing. Various combinations of these exons account for the tremendous heterogeneity of CD44 molecules expressed in different tissues, and in progressing tumor cells. The existence of different isoforms of CD44 suggests a broad spectrum of yet unknown physiologic functions.
摘要
名为CD44的表面蛋白具有极大的异质性，部分原因是翻译后修饰，部分原因是可变剪接。可变剪接发生在编码该蛋白细胞外部分的一个特定区域内。将该可变区域中具有不同“插入片段”的各种cDNA克隆与来自小鼠的基因组克隆序列进行比较，发现至少有十个外显子，可变剪接从中选择序列。这些外显子的各种组合导致了在不同组织以及进展期肿瘤细胞中表达的CD44分子具有极大的异质性。CD44不同异构体的存在表明其具有一系列尚未明确的生理功能。
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本文引用的文献
1
A catalogue of splice junction sequences.剪接连接序列目录。
Nucleic Acids Res. 1982 Jan 22;10(2):459-72. doi: 10.1093/nar/10.2.459.
2
Hormone-responsive expression of an endogenous proviral gene of mouse mammary tumor virus after molecular cloning and gene transfer into cultured cells.小鼠乳腺肿瘤病毒内源性前病毒基因在分子克隆并转入培养细胞后受激素应答的表达
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2038-42. doi: 10.1073/pnas.78.4.2038.
3
Structural heterogeneity of human Pgp-1 and its relationship with p85.人类Pgp-1的结构异质性及其与p85的关系。
Immunogenetics. 1988;27(6):460-4. doi: 10.1007/BF00364434.
4
Structural variants of human T200 glycoprotein (leukocyte-common antigen).人类T200糖蛋白（白细胞共同抗原）的结构变异体
EMBO J. 1987 May;6(5):1251-7. doi: 10.1002/j.1460-2075.1987.tb02361.x.
5
6
Differential exon usage involving an unusual splicing mechanism generates at least eight types of NCAM cDNA in mouse brain.涉及一种异常剪接机制的外显子差异使用在小鼠大脑中产生至少八种类型的NCAM cDNA。
EMBO J. 1989 Feb;8(2):385-92. doi: 10.1002/j.1460-2075.1989.tb03389.x.
7
Isolation of mouse CD44 cDNA: structural features are distinct from the primate cDNA.小鼠CD44 cDNA的分离：结构特征与灵长类cDNA不同。
Proc Natl Acad Sci U S A. 1989 Nov;86(21):8521-5. doi: 10.1073/pnas.86.21.8521.
8
9
Dual role of the CD44 molecule in T cell adhesion and activation.CD44分子在T细胞黏附和激活中的双重作用。
J Immunol. 1989 Oct 15;143(8):2457-63.
10
CD44 contributes to T cell activation.CD44有助于T细胞活化。
J Immunol. 1989 Aug 1;143(3):798-801.
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