Klein H G, Aledort L M, Bouma B N, Hoyer L W, Zimmerman T S, DeMets D L
N Engl J Med. 1977 Apr 28;296(17):959-62. doi: 10.1056/NEJM197704282961702.
To determine the specificity and sensitivity of current technics for detecting carriers of classic hemophilia, three investigators simultaneously tested obligate carriers and noncarriers for immunologic and procoagulant factor VIII activity. Overall correct classification ranged from 72 per cent (36 of 50) to 94 per cent (47 of 50). The maximum accuracy obtained with the same linear-discriminant-function method on all data was 90 per cent (26 of 29) in detecting carriers without misclassifying normal persons as carriers (none of 21). Lowest accuracy by the same technic was 66 per cent (19 of 29) carrier detection while misclassifying 19 per cent (four of 21) normal persons. Precision of testing for both factor VII activity and antigen was high (standard deviations from 0.004 to 0.026 on a log scale). Differences between participants seemed related to laboratory technics rather than to statistical methods. The factor VIII activity/antigen measurement is a valid technic for detecting in the carrier state of hemophilia A.
为了确定当前检测典型血友病携带者技术的特异性和敏感性，三名研究人员同时对肯定携带者和非携带者进行了免疫和促凝血因子VIII活性检测。总体正确分类率从72%（50例中的36例）到94%（50例中的47例）不等。使用相同的线性判别函数方法对所有数据进行检测时，在不将正常人误分类为携带者（21例中无）的情况下，检测携带者的最大准确率为90%（29例中的26例）。使用相同技术检测携带者的最低准确率为66%（29例中的19例），同时将19%（21例中的4例）的正常人误分类。因子VII活性和抗原检测的精密度很高（对数尺度上的标准差为0.004至0.026）。参与者之间的差异似乎与实验室技术有关，而非与统计方法有关。因子VIII活性/抗原检测是检测甲型血友病携带者状态的有效技术。
