Turner G, Brown R C, Weeks I, Butler G E, Creagh F N, Woodhead J S
Department of Medical Biochemistry, University Hospital of Wales, Heath Park, Cardiff, UK.
Ann Clin Biochem. 1993 Mar;30 ( Pt 2):180-5. doi: 10.1177/000456329303000212.
A sensitive immunochemiluminometric assay with a detection limit of 1.1 microU/L was developed for the measurement of urinary growth hormone (UGH). The assay was shown to be specific and precise. There was a good correlation between serum growth hormone (GH) and UGH concentrations in 20 patients with acromegaly and six volunteers following an intravenous injection of recombinant GH. We concluded therefore that UGH measurements appear to provide a satisfactory index of GH secretion. The use of the assay in the investigation of growth disorders was assessed. We studied 11 pre-pubertal children, six of normal stature, and five of short stature, over a 6-month period. Sequential fortnightly measurements of UGH were carried out and height velocity was determined. The children of short stature grew at a slower rate and excreted less GH than the children of normal stature. However, we observed considerable within-individual variability in GH excretion in both groups (CV 22-98%). We therefore recommend that sequential UGH analyses should be carried out and the results interpreted in conjunction with growth measurements. However, further investigations into the renal handling of GH are needed to establish optimum sampling regimes.
我们开发了一种检测限为1.1微单位/升的灵敏免疫化学发光测定法，用于测量尿生长激素（UGH）。该测定法具有特异性和精密度。在20例肢端肥大症患者和6名静脉注射重组生长激素后的志愿者中，血清生长激素（GH）与UGH浓度之间存在良好的相关性。因此，我们得出结论，UGH测量似乎可提供令人满意的GH分泌指标。评估了该测定法在生长障碍研究中的应用。我们在6个月的时间里研究了11名青春期前儿童，其中6名身材正常，5名身材矮小。每两周对UGH进行一次连续测量，并测定身高增长速度。身材矮小的儿童生长速度较慢，GH排泄量比身材正常的儿童少。然而，我们观察到两组儿童的GH排泄量在个体内部都有相当大的变异性（变异系数为22%-98%）。因此，我们建议应进行连续的UGH分析，并结合生长测量结果来解释结果。然而，需要进一步研究GH在肾脏中的处理过程，以确定最佳采样方案。
