Starkey B J, Fry I D
Department of Clinical Biochemistry and Clinical Nutrition, St Lukes Hospital, Guilford, Surrey, UK.
Ann Clin Biochem. 1993 Mar;30 ( Pt 2):186-90. doi: 10.1177/000456329303000213.
A new commercial enzymic kit for urinary oxalate determination has been adapted for use on a centrifugal analyser. It has been evaluated and compared with an established high performance liquid chromatographic (HPLC) procedure developed in our laboratory. Mean recovery of oxalate from urine samples augmented with oxalic acid exceeded 97% by both methods. The precision of the HPLC method was superior to that of the enzymic kit but both methods gave between batch precision values better than CV 12% at low (less than 100 mumol/L) oxalate concentrations and better than CV 7% at higher concentrations (greater than 270 mumol/L). Urinary oxalate values obtained with the new enzymic procedure correlated more closely with HPLC values (r = 0.84) than did values previously obtained using the forerunner of the kit (r = 0.62) which was known to be susceptible to ascorbate interference. No significant interference from ascorbic acid or from high urinary calcium concentrations could be demonstrated using either the improved kit or the HPLC procedure. Its easy adaptation to automated analysers available in most laboratories, coupled to its acceptable analytical performance render the enzymic kit a reasonable alternative to HPLC or other more complex procedures for urinary oxalate analysis.
一种用于测定尿草酸的新型商用酶试剂盒已被适配用于离心分析仪。它已得到评估，并与我们实验室开发的一种既定高效液相色谱（HPLC）方法进行了比较。两种方法对添加草酸的尿液样本中草酸的平均回收率均超过97%。HPLC方法的精密度优于酶试剂盒，但两种方法在低草酸浓度（低于100μmol/L）时批间精密度值均优于CV 12%，在高浓度（高于270μmol/L）时优于CV 7%。与之前使用该试剂盒的前身获得的值（r = 0.62）相比，新酶法获得的尿草酸值与HPLC值的相关性更紧密（r = 0.84），已知该试剂盒的前身易受抗坏血酸干扰。使用改进后的试剂盒或HPLC方法均未显示抗坏血酸或高尿钙浓度有显著干扰。它易于适配大多数实验室现有的自动分析仪，再加上其可接受的分析性能，使得该酶试剂盒成为HPLC或其他更复杂的尿草酸分析方法的合理替代方法。
