Suzuki T, Kumazaki T, Mitsui Y
National Institute of Biosciences and Human-Technology, Agency of Industrial Science and Technology, Ibaraki, Japan.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):823-30. doi: 10.1006/bbrc.1993.1291.
Production of endothelin-1 in the cultured neonatal rat cardiac myocytes was investigated. Studies on the cultured cardiac myocytes with reverse transcription-polymerase chain reaction followed by Southern hybridization indicated that mRNA of ET-1(preproET-1 mRNA) was expressed by cardiac myocytes. ET-1 polypeptide was also detected in the cultured cardiac myocytes. Moreover, ET-1 was secreted into the culture medium by them. These results suggest that the cardiac myocytes, which have been regarded as target cells of ET-1, are able to synthesize and secrete ET-1. Furthermore, there may be a possible autocrine mechanism of ET-1 in the myocytes of a heart.
对培养的新生大鼠心肌细胞中内皮素-1的产生进行了研究。通过逆转录-聚合酶链反应随后进行Southern杂交对培养的心肌细胞进行的研究表明，ET-1的mRNA（前原ET-1 mRNA）由心肌细胞表达。在培养的心肌细胞中也检测到了ET-1多肽。此外，ET-1由它们分泌到培养基中。这些结果表明，一直被视为ET-1靶细胞的心肌细胞能够合成和分泌ET-1。此外，在心脏的心肌细胞中可能存在ET-1的自分泌机制。
