Yamagishi S, Hsu C C, Kobayashi K, Yamamoto H
Department of Biochemistry, Kanazawa University School of Medicine, Japan.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):840-6. doi: 10.1006/bbrc.1993.1293.
The influence of endothelial cells on the growth of vascular pericytes was investigated using human umbilical endothelial cell and bovine retinal pericyte co-culture systems. When pericytes were cultured with endothelial cells, the number of viable pericytes increased significantly, regardless of the presence or absence of physical contact between the two cell types, indicating that endothelial cells can induce proliferation of pericytes through certain secreted factor(s). Evidence that endothelin 1 mediates this action was obtained: endothelin 1 added to the medium was able to substitute for the feeder layer of endothelial cells, and an antiserum against endothelin 1 could neutralize the ability of endothelial cells to promote pericyte growth. RNA blot analysis revealed that pericytes express mRNA for type-A endothelin receptor, a high affinity receptor of endothelin 1.
利用人脐静脉内皮细胞和牛视网膜周细胞共培养系统，研究了内皮细胞对血管周细胞生长的影响。当周细胞与内皮细胞共培养时，无论两种细胞类型之间是否存在物理接触，存活周细胞的数量均显著增加，这表明内皮细胞可通过某些分泌因子诱导周细胞增殖。有证据表明内皮素-1介导了这一作用：添加到培养基中的内皮素-1能够替代内皮细胞饲养层，且抗内皮素-1抗血清可中和内皮细胞促进周细胞生长的能力。RNA印迹分析显示，周细胞表达A型内皮素受体的mRNA，A型内皮素受体是内皮素-1的高亲和力受体。
