Gonzalez P, Rao P V, Zigler J S
Laboratory of Mechanisms of Ocular Diseases, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):902-7. doi: 10.1006/bbrc.1993.1302.
Zeta-crystallin is an enzyme-crystallin highly expressed in the lens of some hystricomorph rodents and camels. It has been shown to have a novel NADPH: quinone oxidoreductase activity and is present at enzymatic levels in a variety of tissues from various mammals. We report here the cDNA cloning of zeta-crystallin from a human liver library. One clone with the complete open reading frame was obtained. Ten nucleotides of the 5' and 796 of the 3' nontranslated regions are present in the clone including two possible polyadenylation signals. The deduced amino acid sequence is 328 residues long with a calculated molecular mass of 34910 daltons and isoelectric point of 8.73. It shows 84% identity with the guinea pig protein.
ζ-晶体蛋白是一种在某些豪猪型啮齿动物和骆驼的晶状体中高度表达的酶晶体蛋白。已证明它具有一种新型的NADPH：醌氧化还原酶活性，并且在来自各种哺乳动物的多种组织中以酶水平存在。我们在此报告从人肝文库中克隆ζ-晶体蛋白的cDNA。获得了一个具有完整开放阅读框的克隆。该克隆中存在5'端的10个核苷酸和3'非翻译区的796个核苷酸，包括两个可能的聚腺苷酸化信号。推导的氨基酸序列长328个残基，计算分子量为34910道尔顿，等电点为8.73。它与豚鼠蛋白的同一性为84%。
