Amiche M, Ducancel F, Lajeunesse E, Boulain J C, Ménez A, Nicolas P
Laboratoire de bioactivation des peptides, Université Paris 7, France.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):983-90. doi: 10.1006/bbrc.1993.1314.
Adenoregulin has recently been isolated from Phyllomedusa skin as a 33 amino acid residues peptide which enhanced binding of agonists to the A1 adenosine receptor. In order to study the structure of the precursor of adenoregulin we constructed a cDNA library from mRNAs extracted from the skin of Phyllomedusa bicolor. We detected the complete nucleotide sequence of a cDNA encoding the adenoregulin biosynthetic precursor. The deduced sequence of the precursor is 81 amino acids long, exhibits a putative signal sequence at the NH2 terminus and contains a single copy of the biologically active peptide at the COOH terminus. Structural and conformational homologies that are observed between adenoregulin and the dermaseptins, antimicrobial peptides exhibiting strong membranolytic activities against various pathogenic agents, suggest that adenoregulin is an additional member of the growing family of cytotropic antimicrobial peptides that allow vertebrate animals to defend themselves against microorganisms. As such, the adenosine receptor regulating activity of adenoregulin could be due to its ability to interact with and disrupt membranes lipid bilayers.
最近从双色叶蛙皮肤中分离出腺调节蛋白，它是一种由33个氨基酸残基组成的肽，可增强激动剂与A1腺苷受体的结合。为了研究腺调节蛋白前体的结构，我们从双色叶蛙皮肤中提取的mRNA构建了一个cDNA文库。我们检测到了编码腺调节蛋白生物合成前体的cDNA的完整核苷酸序列。前体的推导序列长81个氨基酸，在NH2末端显示出一个假定的信号序列，在COOH末端含有一个生物活性肽的单拷贝。腺调节蛋白与皮肤防御素之间观察到的结构和构象同源性，皮肤防御素是对各种病原体具有强大膜溶解活性的抗菌肽，这表明腺调节蛋白是不断增加的亲细胞抗菌肽家族的又一个成员，这些抗菌肽使脊椎动物能够抵御微生物。因此，腺调节蛋白的腺苷受体调节活性可能是由于其与膜脂质双层相互作用并破坏膜脂质双层的能力。
