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通过酶联免疫吸附测定法检测实验性感染马埃里希体的马匹中的体液抗原和抗体。

Detection of humoral antigen and antibody by enzyme-linked immunosorbent assay in horses with experimentally induced Ehrlichia equi infection.

作者信息

Corstvet R E, Gaunt S D, Karns P A, McBride J W, Battistini R A, Mauterer L A, Austin F W

机构信息

Department of Veterinary Science, LSU Agricultural Center, Baton Rouge.

出版信息

J Vet Diagn Invest. 1993 Jan;5(1):37-9. doi: 10.1177/104063879300500109.

Abstract

An enzyme-linked immunosorbent assay (ELISA) was used to detect antigen in plasma and antibody in serum of 3 horses inoculated with Ehrlichia equi. Clinical signs, including rectal temperature, were correlated with the antigen and antibody detection. ELISA was very efficient in detection of serum antibody. Antigen detection using monoclonal antibodies to E. equi and ELISA should be considered as a diagnostic method.

摘要

采用酶联免疫吸附测定(ELISA)检测3匹接种马埃里希体的马的血浆抗原和血清抗体。将包括直肠温度在内的临床症状与抗原和抗体检测结果进行关联。ELISA在检测血清抗体方面非常有效。使用抗马埃里希体单克隆抗体和ELISA进行抗原检测应被视为一种诊断方法。

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