Zaman G J, Versantvoort C H, Smit J J, Eijdems E W, de Haas M, Smith A J, Broxterman H J, Mulder N H, de Vries E G, Baas F
Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam.
Cancer Res. 1993 Apr 15;53(8):1747-50.
Human cells can become multidrug resistant (MDR) by an increase in the activity of the MDR1 P-glycoprotein or by other, as yet unknown mechanisms, referred to as non-P-glycoprotein mediated MDR (non-Pgp MDR). S. P. C. Cole et al. [Science (Washington DC), 258: 1650-1654, 1992] recently reported that in two cell lines non-Pgp MDR was associated with the overexpression of a new putative membrane transporter gene, MRP. Using an RNase protection assay we have analyzed the expression of MRP in non-Pgp MDR sublines of the human lung cancer cell lines SW-1573 (non-small cell lung cancer) and GLC4 (small cell lung cancer). In all of ten SW-1573 derived lines examined the MRP mRNA level was equal to that in the parental line, whereas MRP was 25-fold overexpressed in a resistant subline of GLC4. We conclude that overexpression of MRP cannot account for all forms of non-Pgp MDR.
人类细胞可通过增加MDR1 P-糖蛋白的活性或通过其他尚未明确的机制（称为非P-糖蛋白介导的多药耐药，即non-Pgp MDR）而产生多药耐药性。S. P. C. 科尔等人[《科学》（华盛顿特区），258: 1650 - 1654，1992]最近报道，在两个细胞系中，non-Pgp MDR与一种新的假定膜转运蛋白基因MRP的过表达有关。我们使用核糖核酸酶保护分析方法，分析了MRP在人肺癌细胞系SW - 1573（非小细胞肺癌）和GLC4（小细胞肺癌）的非Pgp MDR亚系中的表达情况。在检测的所有10个源自SW - 1573的细胞系中，MRP mRNA水平与亲代细胞系相同，而在GLC4的一个耐药亚系中，MRP过表达了25倍。我们得出结论，MRP的过表达不能解释所有形式的non-Pgp MDR。
