通过超滤/透析法测定犬血浆中游离皮质醇的百分比。

Determination of the percentage of free cortisol in plasma in the dog by ultrafiltration/dialysis.

作者信息

Meyer H P, Rothuizen J

机构信息

Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.

出版信息

Domest Anim Endocrinol. 1993 Jan;10(1):45-53. doi: 10.1016/0739-7240(93)90007-x.

DOI:10.1016/0739-7240(93)90007-x

PMID:8467645

Abstract

The current methods for measurement of free cortisol are hampered by methodological drawbacks including large sample requirement, length of the procedure, and overestimation or underestimation of free cortisol. We report a technique combining ultrafiltration and dialysis, which could largely overcome these drawbacks, for measurement of the per cent free cortisol in plasma in the dog. This is a modification of the method of Hammond et al. for the determination of sex steroids in man. We found a good positive correlation (rs = 0.87) with equilibrium dialysis, which is considered the golden standard in free cortisol measurements. The 2.5th and 97.5th percentiles of values of free cortisol measured in plasma of 40 healthy household dogs were 5-20 nmol/l or 5.1-11.7% of the total cortisol concentration.

摘要

目前用于测量游离皮质醇的方法存在方法学上的缺陷，包括需要大量样本、检测过程耗时，以及游离皮质醇的高估或低估。我们报告了一种结合超滤和透析的技术，该技术可以很大程度上克服这些缺陷，用于测量犬血浆中游离皮质醇的百分比。这是对哈蒙德等人用于测定人体性激素方法的改进。我们发现该技术与平衡透析法有良好的正相关性（rs = 0.87），平衡透析法被认为是游离皮质醇测量的金标准。在40只健康家养犬的血浆中测量的游离皮质醇值的第2.5百分位数和第97.5百分位数分别为5 - 20 nmol/l或总皮质醇浓度的5.1 - 11.7%。