• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酿酒酵母中ARG4减数分裂双链断裂位置和顺式控制的数量

The control in cis of the position and the amount of the ARG4 meiotic double-strand break of Saccharomyces cerevisiae.

作者信息

de Massy B, Nicolas A

机构信息

Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France.

出版信息

EMBO J. 1993 Apr;12(4):1459-66. doi: 10.1002/j.1460-2075.1993.tb05789.x.

DOI:10.1002/j.1460-2075.1993.tb05789.x
PMID:8467798
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC413357/
Abstract

During meiosis, a transient DNA double-strand break (DSB) occurs in the promoter region (positions -200/-185) of the Saccharomyces cerevisiae ARG4 gene and is a likely intermediate in the initiation of meiotic gene conversion events in this region. We report here a functional analysis of the ARG4 DSB based on the study of various deletions in this chromosomal region. We have identified several cis-acting elements located within the -465/+3 region of the ARG4 promoter that control the formation of this DSB. The -465/-317 region includes a transcription terminator and is necessary for a normal amount of ARG4 DSB, but not for its positioning. The -316/-140 region can be replaced by an unrelated DNA sequence where a meiotic DSB then occurs, suggesting that the site of DSB is not sequence-specific, but is positioned at a fixed distance from the adjacent -139/+3 region. Also, in all strains constructed, the amount of meiotic DSB is correlated with the frequency of gene conversion in ARG4, which provides a strong argument for the initiation of gene conversion by a DSB in this region of the yeast genome.

摘要

在减数分裂过程中，酿酒酵母ARG4基因的启动子区域（位置-200/-185）会发生短暂的DNA双链断裂（DSB），这可能是该区域减数分裂基因转换事件起始过程中的一个中间体。我们在此基于对该染色体区域各种缺失的研究报告了ARG4 DSB的功能分析。我们已经鉴定出位于ARG4启动子-465/+3区域内的几个顺式作用元件，它们控制着这种DSB的形成。-465/-317区域包含一个转录终止子，对于正常数量的ARG4 DSB是必需的，但对于其定位不是必需的。-316/-140区域可以被一个不相关的DNA序列取代，在此处会发生减数分裂DSB，这表明DSB的位点不是序列特异性的，而是位于距相邻的-139/+3区域固定距离处。此外，在构建的所有菌株中，减数分裂DSB的数量与ARG4中基因转换的频率相关，这为酵母基因组该区域中由DSB引发基因转换提供了有力证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/8b0d2dc4b4a7/emboj00076-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/b54f14e5a7fc/emboj00076-0204-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/101cc873d7ab/emboj00076-0204-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/fa29745dcde2/emboj00076-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/9f3e2c34551a/emboj00076-0205-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/3a54b4e32895/emboj00076-0205-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/8b0d2dc4b4a7/emboj00076-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/b54f14e5a7fc/emboj00076-0204-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/101cc873d7ab/emboj00076-0204-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/fa29745dcde2/emboj00076-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/9f3e2c34551a/emboj00076-0205-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/3a54b4e32895/emboj00076-0205-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80e5/413357/8b0d2dc4b4a7/emboj00076-0206-a.jpg

相似文献

1
The control in cis of the position and the amount of the ARG4 meiotic double-strand break of Saccharomyces cerevisiae.酿酒酵母中ARG4减数分裂双链断裂位置和顺式控制的数量
EMBO J. 1993 Apr;12(4):1459-66. doi: 10.1002/j.1460-2075.1993.tb05789.x.
2
The Saccharomyces cerevisiae ARG4 initiator of meiotic gene conversion and its associated double-strand DNA breaks can be inhibited by transcriptional interference.酿酒酵母减数分裂基因转换的起始因子ARG4及其相关的双链DNA断裂可受到转录干扰的抑制。
Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12068-72. doi: 10.1073/pnas.89.24.12068.
3
Competitive inactivation of a double-strand DNA break site involves parallel suppression of meiosis-induced changes in chromatin configuration.双链DNA断裂位点的竞争性失活涉及对减数分裂诱导的染色质构型变化的平行抑制。
Nucleic Acids Res. 1999 May 15;27(10):2175-80. doi: 10.1093/nar/27.10.2175.
4
Factors that affect the location and frequency of meiosis-induced double-strand breaks in Saccharomyces cerevisiae.影响酿酒酵母中减数分裂诱导双链断裂的位置和频率的因素。
Genetics. 1995 May;140(1):55-66. doi: 10.1093/genetics/140.1.55.
5
A poly(dA.dT) tract is a component of the recombination initiation site at the ARG4 locus in Saccharomyces cerevisiae.聚（dA.dT）序列是酿酒酵母中ARG4基因座重组起始位点的一个组成部分。
Mol Cell Biol. 1991 Jan;11(1):322-8. doi: 10.1128/mcb.11.1.322-328.1991.
6
Extensive 3'-overhanging, single-stranded DNA associated with the meiosis-specific double-strand breaks at the ARG4 recombination initiation site.与ARG4重组起始位点处减数分裂特异性双链断裂相关的广泛3'突出端单链DNA。
Cell. 1991 Mar 22;64(6):1155-61. doi: 10.1016/0092-8674(91)90270-9.
7
Sensing of DNA non-homology lowers the initiation of meiotic recombination in yeast.对DNA非同源性的感知会降低酵母减数分裂重组的起始。
Genes Cells. 1996 Jul;1(7):645-61. doi: 10.1046/j.1365-2443.1996.00256.x.
8
Double-strand breaks at an initiation site for meiotic gene conversion.减数分裂基因转换起始位点处的双链断裂。
Nature. 1989 Mar 2;338(6210):87-90. doi: 10.1038/338087a0.
9
Decreasing gradients of gene conversion on both sides of the initiation site for meiotic recombination at the ARG4 locus in yeast.酵母中ARG4基因座减数分裂重组起始位点两侧基因转换梯度的降低。
Genetics. 1990 Dec;126(4):813-22. doi: 10.1093/genetics/126.4.813.
10
An initiation site for meiotic gene conversion in the yeast Saccharomyces cerevisiae.酿酒酵母中减数分裂基因转换的起始位点。
Nature. 1989 Mar 2;338(6210):35-9. doi: 10.1038/338035a0.

引用本文的文献

1
Advances Towards How Meiotic Recombination Is Initiated: A Comparative View and Perspectives for Plant Meiosis Research.减数分裂重组起始的研究进展：比较观点与植物减数分裂研究展望。
Int J Mol Sci. 2019 Sep 23;20(19):4718. doi: 10.3390/ijms20194718.
2
A Critical Assessment of 60 Years of Maize Intragenic Recombination.对60年玉米基因内重组的批判性评估。
Front Plant Sci. 2018 Oct 29;9:1560. doi: 10.3389/fpls.2018.01560. eCollection 2018.
3
Budding yeast ATM/ATR control meiotic double-strand break (DSB) levels by down-regulating Rec114, an essential component of the DSB-machinery.

本文引用的文献

1
The double-strand-break repair model for recombination.用于重组的双链断裂修复模型。
Cell. 1983 May;33(1):25-35. doi: 10.1016/0092-8674(83)90331-8.
2
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
3
Genomic sequencing.基因组测序
芽殖酵母 ATM/ATR 通过下调减数分裂双链断裂（DSB）机制的必需组件 Rec114 来控制 DSB 水平。
PLoS Genet. 2013 Jun;9(6):e1003545. doi: 10.1371/journal.pgen.1003545. Epub 2013 Jun 27.
4
Trans-regulation of mouse meiotic recombination hotspots by Rcr1.Rcr1对小鼠减数分裂重组热点的反式调控。
PLoS Biol. 2009 Feb 17;7(2):e36. doi: 10.1371/journal.pbio.1000036.
5
Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae.对酿酒酵母减数分裂重组过程中形成的碱基错配和四碱基环的体内修复所涉及蛋白质的分析。
Genetics. 2006 Jul;173(3):1223-39. doi: 10.1534/genetics.106.055616. Epub 2006 May 15.
6
Persistence and loss of meiotic recombination hotspots.减数分裂重组热点的持续性与丧失
Genetics. 2005 Apr;169(4):2319-33. doi: 10.1534/genetics.104.034363. Epub 2005 Jan 31.
7
MuDR transposase increases the frequency of meiotic crossovers in the vicinity of a Mu insertion in the maize a1 gene.MuDR转座酶增加了玉米a1基因中Mu插入位点附近减数分裂交叉的频率。
Genetics. 2005 Feb;169(2):917-29. doi: 10.1534/genetics.104.035089. Epub 2004 Oct 16.
8
A test of the CoHR motif associated with meiotic double-strand breaks in Saccharomyces cerevisiae.一项关于与酿酒酵母减数分裂双链断裂相关的CoHR基序的测试。
EMBO Rep. 2004 Jan;5(1):41-6. doi: 10.1038/sj.embor.7400050.
9
Properties of natural double-strand-break sites at a recombination hotspot in Saccharomyces cerevisiae.酿酒酵母中一个重组热点处天然双链断裂位点的特性。
Genetics. 2003 Sep;165(1):101-14. doi: 10.1093/genetics/165.1.101.
10
(CA/TG) microsatellite sequences escape the inhibition of recombination by mismatch repair in Saccharomyces cerevisiae.（CA/TG）微卫星序列在酿酒酵母中可逃避错配修复对重组的抑制作用。
Genetics. 2001 Dec;159(4):1539-45. doi: 10.1093/genetics/159.4.1539.
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5. doi: 10.1073/pnas.81.7.1991.
4
A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae.一种对酿酒酵母交配型转换至关重要的位点特异性内切核酸酶。
Cell. 1983 Nov;35(1):167-74. doi: 10.1016/0092-8674(83)90219-2.
5
Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus.酵母交配型盒式结构的同宗配合转换由MAT基因座中的双链切割引发。
Cell. 1982 Nov;31(1):183-92. doi: 10.1016/0092-8674(82)90418-4.
6
Yeast transformation: a model system for the study of recombination.酵母转化：用于重组研究的模型系统。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8. doi: 10.1073/pnas.78.10.6354.
7
A transcription map of a yeast centromere plasmid: unexpected transcripts and altered gene expression.酵母着丝粒质粒的转录图谱：意外的转录本与基因表达的改变
Nucleic Acids Res. 1985 Dec 9;13(23):8487-506. doi: 10.1093/nar/13.23.8487.
8
Transposition of an intron in yeast mitochondria requires a protein encoded by that intron.酵母线粒体中一个内含子的转座需要该内含子编码的一种蛋白质。
Cell. 1985 Jun;41(2):395-402. doi: 10.1016/s0092-8674(85)80012-x.
9
One-step gene replacement in yeast by cotransformation.通过共转化在酵母中进行一步基因置换。
Gene. 1985;36(1-2):87-95. doi: 10.1016/0378-1119(85)90072-1.
10
DNA double-chain breaks in recombination of phage lambda and of yeast.噬菌体λ和酵母重组过程中的DNA双链断裂
Annu Rev Genet. 1988;22:169-97. doi: 10.1146/annurev.ge.22.120188.001125.