Blackburn B J, Buist R J, Hudspeth C, Novak M
Department of Chemistry, University of Winnipeg, Manitoba, Canada.
Int J Parasitol. 1993 Feb;23(1):95-103. doi: 10.1016/0020-7519(93)90102-5.
31P NMR in vivo spectra of mouse livers infected with Hymenolepis microstoma for 130 or 265 days showed modifications in phosphorus-containing metabolite ratios when compared to those of normal liver. After 130 days of infection the metabolite ratio of inorganic phosphate (Pi)/beta ATP significantly increased whereas that of phosphocreatine (PCr)/beta ATP significantly decreased. In older, 265 day infections, the increase in Pi/beta ATP and decrease in PCr/beta ATP persisted. Changes in the group infected for 130 days were accompanied by lowered pH. Analysis of liver extracts from mice with 130-day-old. H. microstoma revealed significantly lower concentrations of Pi, ATP and ADP compounds. In those from mice infected for 265 days the concentration of Pi remained low whereas concentrations of ATP and ADP increased to levels in between those of controls and the 130-day-old infection. In addition, levels of phosphorylethanolamine (PE) and of an unknown metabolite significantly increased in this latter group. Worm extracts contained high levels of glycerophosphorylcholine (GPC), Pi, fructose 1,6-diphosphate (FDP), PE, diphosphodiesters (DPDE), phosphorylcholine (PC) and glycerolphosphorylethanolamine (GPE) in order of declining concentrations, respectively.
感染微小膜壳绦虫130天或265天的小鼠肝脏的31P NMR体内光谱显示，与正常肝脏相比，含磷代谢物比率发生了变化。感染130天后，无机磷酸盐（Pi）/β-ATP的代谢物比率显著增加，而磷酸肌酸（PCr）/β-ATP的比率显著降低。在感染265天的老龄小鼠中，Pi/β-ATP的增加和PCr/β-ATP的降低仍然存在。感染130天的组的变化伴随着pH值降低。对感染微小膜壳绦虫130天的小鼠肝脏提取物的分析显示，Pi、ATP和ADP化合物的浓度显著降低。在感染265天的小鼠肝脏提取物中，Pi的浓度仍然很低，而ATP和ADP的浓度增加到介于对照组和感染130天的小鼠之间的水平。此外，后一组中磷酸乙醇胺（PE）和一种未知代谢物的水平显著增加。蠕虫提取物中甘油磷酸胆碱（GPC）、Pi、果糖1,6-二磷酸（FDP）、PE、二磷酸二酯（DPDE）、磷酸胆碱（PC）和甘油磷酸乙醇胺（GPE）的含量依次降低。
