组织中挥发性 C2 卤代乙烷和卤代乙烯的分析：样品制备与提取

Analyses of volatile C2 haloethanes and haloethenes in tissues: sample preparation and extraction.

作者信息

Chen X M, Dallas C E, Muralidhara S, Srivatsan V, Bruckner J V

机构信息

Department of Pharmacology and Toxicology, College of Pharmacy, University of Georgia, Athens 30602-2356.

出版信息

J Chromatogr. 1993 Feb 26;612(2):199-208. doi: 10.1016/0378-4347(93)80164-y.

DOI:10.1016/0378-4347(93)80164-y

PMID:8468377

Abstract

A tissue extraction procedure was developed which minimized loss of readily volatilizable compounds for subsequent quantification by headspace gas chromatography, and evaluated for perchloroethylene (PER), 1,1,1-trichloroethane, 1,1,2,2-tetrachloroethane, and 1,1,2-trichloroethylene. Of the procedures evaluated, joint isooctane and saline tissue homogenization had the most efficient recovery, ranging from 73 to 104% for the four halocarbons from seven different rat tissues. PER concentrations were also determined in tissues of rats following in vivo halocarbon administration. Recovery did not appear to be tissue-dependent, but did vary somewhat with test chemical, with the least volatile, most lipophilic compounds exhibiting the highest recovery.

摘要

开发了一种组织提取程序，该程序可最大程度减少易于挥发的化合物的损失，以便随后通过顶空气相色谱法进行定量分析，并针对全氯乙烯（PER）、1,1,1-三氯乙烷、1,1,2,2-四氯乙烷和1,1,2-三氯乙烯进行了评估。在所评估的程序中，异辛烷和生理盐水联合组织匀浆法具有最高的回收率，七种不同大鼠组织中的四种卤代烃的回收率在73%至104%之间。还测定了体内给予卤代烃后大鼠组织中的PER浓度。回收率似乎不依赖于组织，但会因测试化学品的不同而有所变化，挥发性最小、亲脂性最强的化合物回收率最高。