通过液相色谱与紫外可见分光光度法联用提高利尿剂筛查的检测限

Improved detection limits for screening of diuretics by coupled liquid chromatography and ultraviolet-visible spectrophotometry.

作者信息

Campíns-Falcó P, Herráez-Hernández R, Sevillano-Cabeza A

机构信息

Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot, Spain.

出版信息

J Chromatogr. 1993 Feb 26;612(2):245-51. doi: 10.1016/0378-4347(93)80170-9.

DOI:10.1016/0378-4347(93)80170-9

PMID:8468382

Abstract

Experimental conditions have been studied in order to improve the sensitivity for the analysis of diuretics and probenecid in urine samples by high-performance liquid chromatography with ultraviolet detection. Sample clean-up and chromatographic parameters have been optimized to obtain a suitable sensitivity for the detection or quantification of each diuretic using an HP-Hypersil ODS-C18 column (5 microns, 250 mm x 4 mm I.D.), taking into account the pharmacological properties of each compound. The reliability of this method was tested by analysing urine samples after a minimum single-dose administration of chlorthalidone and probenecid.

摘要

为了提高高效液相色谱-紫外检测法分析尿样中利尿剂和丙磺舒的灵敏度，对实验条件进行了研究。考虑到每种化合物的药理特性，已对样品净化和色谱参数进行了优化，以使用HP-Hypersil ODS-C18柱（5微米，250毫米×4毫米内径）获得检测或定量每种利尿剂的合适灵敏度。通过分析单次最小剂量服用氯噻酮和丙磺舒后的尿样，对该方法的可靠性进行了测试。