氯贝丁酯可提高大鼠肝脏中三羧酸循环的酶活性。

Clofibrate elevates enzyme activities of the tricarboxylic acid cycle in rat liver.

作者信息

Prager C, Schön H J, Nikfardjam M, Schmid D, Untersalmberger M, Kremser K, Kramar R

机构信息

Department of Medical Chemistry, University of Vienna, Austria.

出版信息

J Lipid Res. 1993 Mar;34(3):359-64.

PMID:8468521

Abstract

Activities of the tricarboxylic acid cycle enzymes were measured in subcellular fractions of liver from rats that had been fed clofibrate for 3 weeks. Large changes in these activities per gram tissue were found in the large particle fraction, which also showed an increase in total protein concentration of 76% under clofibrate treatment. The three regulatory enzymes of the cycle, namely citrate synthase, NAD(+)-linked isocitrate dehydrogenase, and 2-oxoglutarate dehydrogenase were significantly enhanced by 24% (P < 0.02), 54% (P < 0.02), and 153% (P < 0.005), respectively. Fumarase and malate dehydrogenase rose by 71% (P < 0.005) and 95% (P < 0.02), whereas succinate dehydrogenase remained unchanged. Enhancement of the citrate synthase, NAD-isocitrate dehydrogenase, and 2-oxoglutarate dehydrogenase may play a role in decreasing intracellular availability of acetyl-CoA for lipid metabolism.

摘要

在喂食氯贝丁酯3周的大鼠肝脏亚细胞组分中，测定了三羧酸循环酶的活性。在大颗粒组分中发现，每克组织中这些酶的活性有很大变化，在氯贝丁酯处理下，该组分的总蛋白浓度也增加了76%。循环的三种调节酶，即柠檬酸合酶、NAD(+)-连接的异柠檬酸脱氢酶和2-氧代戊二酸脱氢酶分别显著增强了24%（P < 0.02）、54%（P < 0.02）和153%（P < 0.005）。延胡索酸酶和苹果酸脱氢酶分别升高了71%（P < 0.005）和95%（P < 0.02），而琥珀酸脱氢酶则保持不变。柠檬酸合酶、NAD-异柠檬酸脱氢酶和2-氧代戊二酸脱氢酶的增强可能在降低细胞内用于脂质代谢的乙酰辅酶A可用性方面发挥作用。