Scoles G A, Dickson S L, Blackmore M S
Vector Biology Laboratories, University of Notre Dame, IN 46556.
J Am Mosq Control Assoc. 1993 Mar;9(1):88-90.
Both Aedes sierrensis and Dirofilaria immitis have recently become established in Utah. We evaluated the vector potential of this Aedes sierrensis strain using a new technique for detecting Dirofilaria immitis in individual mosquitoes. Survival of Aedes sierrensis females after bloodfeeding did not differ from that of Ae. triseriatus but infective Ae. sierrensis produced significantly more L3 nematodes. This observation and epidemiological data support the hypothesis that Ae. sierrensis is the vector of canine heartworm in Utah. Infectivity was determined by counting infective-stage parasites that migrated into the medium after individual mosquitoes were decapitated or crushed in the wells of tissue culture plates. Complete recovery of infective-stage nematodes was attained in 60-74% of the mosquitoes and 77-93% of all L3 were collected with this technique. There were few false negatives. High recovery rates (mean = 89%) were also obtained for mosquitoes treated en masse.
锡耶拉伊蚊和犬恶丝虫最近在犹他州定殖。我们使用一种检测单个蚊子体内犬恶丝虫的新技术，评估了锡耶拉伊蚊品系的媒介潜能。吸血后锡耶拉伊蚊雌蚊的存活率与三带喙库蚊没有差异，但感染性锡耶拉伊蚊产生的L3期线虫显著更多。这一观察结果和流行病学数据支持了锡耶拉伊蚊是犹他州犬心丝虫媒介的假说。通过计数在组织培养板孔中单个蚊子被断头或碾碎后迁移到培养基中的感染期寄生虫来确定感染性。60%-74%的蚊子中感染期线虫完全回收，并且用该技术收集了所有L3期线虫的77%-93%。假阴性很少。对成批处理的蚊子也获得了高回收率（平均=89%）。
