Amore A, Cavallo F, Bocchietto E, Bussolino F, Gianoglio B, Peruzzi L, Porcellini M G, Coppo R
Nephrology and Dialysis Department, Regina Margherita Children's Hospital, Turin, Italy.
Kidney Int Suppl. 1993 Jan;39:S41-6.
We previously demonstrated that gliadin, a lectinic component of gluten, induces IgA mesangial deposits in orally immunized mice, binds in vitro polymeric IgA and cultured rat mesangial cells modulating their arachidonic acid metabolism. We investigated the effects of gliadin and other environmental lectins on some mesangial cell functions, including synthesis and release of cytokines and lipid mediators. Several lectins, particularly gliadin, affected the mRNA expression of c-myc and c-fos, two proto-oncogenes involved in the transcriptional enhancement of the gene cascade, which are markers of cell growth, differentiation and mitosis. Lectins modulated the ability of cultured rat mesangial cells to express mRNA for cytokines involved in the inflammation and in the regulation of the immune response. TNF-alpha and IL-6 mRNA transcription were enhanced by gliadin and other lectins, and TNF release was variably increased. Conversely, IL-1 production was less affected or slightly depressed. PAF production was not detectable while PGE2 was generally reduced and TXB2 enhanced. Gliadin was one of the lectins most active on the mesangial cells, and its effects were reversed by the addition of N-Acetylglucosamine, a sugar specific for some lectinic bindings, suggesting a carbohydrate interaction. The effects of the various lectins were distinct and only partially convergent, ruling out an aspecific mesangial cell activation. These data suggest that lectins might interfere with mesangial cell functions and modify the mesangial cell homeostasis.
我们之前证明，麦醇溶蛋白是麸质中的一种凝集素成分，可在经口免疫的小鼠中诱导IgA系膜沉积物，在体外与聚合IgA结合，并与培养的大鼠系膜细胞结合，调节其花生四烯酸代谢。我们研究了麦醇溶蛋白和其他环境凝集素对一些系膜细胞功能的影响，包括细胞因子和脂质介质的合成与释放。几种凝集素，特别是麦醇溶蛋白，影响了c-myc和c-fos的mRNA表达，这两个原癌基因参与基因级联的转录增强，是细胞生长、分化和有丝分裂的标志物。凝集素调节培养的大鼠系膜细胞表达参与炎症和免疫反应调节的细胞因子mRNA的能力。麦醇溶蛋白和其他凝集素增强了TNF-α和IL-6的mRNA转录，TNF释放也有不同程度的增加。相反，IL-1的产生受影响较小或略有降低。未检测到PAF的产生，而PGE2通常减少，TXB2增加。麦醇溶蛋白是对系膜细胞最具活性的凝集素之一，加入N-乙酰葡糖胺（一种对某些凝集素结合具有特异性的糖）可逆转其作用，提示存在碳水化合物相互作用。各种凝集素的作用是不同的，且只有部分趋同，排除了非特异性系膜细胞激活。这些数据表明，凝集素可能会干扰系膜细胞功能并改变系膜细胞的内环境稳定。
