Rifai A, Sakai H, Yagame M
Department of Pathology, Brown University School of Medicine, Providence, Rhode Island.
Kidney Int Suppl. 1993 Jan;39:S95-9.
The gene transcription of 5-lipoxygenase (5-LO) and 5-lipoxygenase-activating protein (FLAP) in renal tissue of patients with IgA nephropathy and mesangial proliferative glomerulonephritis were analyzed by amplification of reverse transcribed mRNA with polymerase chain reaction (PCR). The level of expression was determined by analysis of the PCR products of 5-LO, FLAP, and beta-actin. Forty percent of the patients expressed both 5-LO and FLAP. Relative to beta-actin, FLAP expression (0.42 +/- 0.21) was higher than 5-LO (0.14 +/- 0.10). Comparison of clinical data showed that patients who expressed 5-LO and FLAP had a lower glomerular filtration rate and an increased level of blood urea nitrogen, serum creatinine, and proteinuria. The results suggest arachidonic acid metabolism by inflammatory cells in renal tissue may play an important role in human glomerulonephritis.
采用聚合酶链反应（PCR）扩增逆转录mRNA的方法，分析IgA肾病和系膜增生性肾小球肾炎患者肾组织中5-脂氧合酶（5-LO）和5-脂氧合酶激活蛋白（FLAP）的基因转录情况。通过分析5-LO、FLAP和β-肌动蛋白的PCR产物来确定表达水平。40%的患者同时表达5-LO和FLAP。相对于β-肌动蛋白，FLAP的表达水平（0.42±0.21）高于5-LO（0.14±0.10）。临床资料比较显示，表达5-LO和FLAP的患者肾小球滤过率较低，血尿素氮、血清肌酐和蛋白尿水平升高。结果提示，肾组织中炎症细胞的花生四烯酸代谢可能在人类肾小球肾炎中起重要作用。
