Steinhilber D, Rådmark O, Samuelsson B
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):1083-90. doi: 10.1006/bbrc.1993.1736.
5-Lipoxygenase (5-LO) activity in differentiating HL-60 cells was upregulated by a heat stable protein in human serum. Hematopoietic cytokines were evaluated for this effect on 5-lipoxygenase activity, none of the compounds tested could replace serum. However, TNF alpha or GM-CSF were able to augment the effect of heat treated serum, giving 5-LO activities that (per cell) were higher than for granulocytes isolated from peripheral blood. The serum factor primarily upregulated 5-LO activity. The amounts of 5-LO protein, or mRNA for 5-LO or 5-lipoxygenase activating protein (FLAP), were less affected.
人血清中的一种热稳定蛋白可上调分化型HL-60细胞中的5-脂氧合酶（5-LO）活性。对造血细胞因子对5-脂氧合酶活性的这种作用进行了评估，所测试的化合物均不能替代血清。然而，肿瘤坏死因子α或粒细胞巨噬细胞集落刺激因子（GM-CSF）能够增强热处理血清的作用，使（每细胞）5-LO活性高于从外周血分离的粒细胞。血清因子主要上调5-LO活性。5-LO蛋白的量，或5-LO或5-脂氧合酶激活蛋白（FLAP）的mRNA受影响较小。
